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Molecules 2016, 21(9), 1138; doi:10.3390/molecules21091138

Screening for Neuraminidase Inhibitory Activity in Traditional Chinese Medicines Used to Treat Influenza

1
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
2
College of Pharmacy and Chemistry, Dali University, Yunnan 671000, China
3
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
4
Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 27 July 2016 / Revised: 16 August 2016 / Accepted: 23 August 2016 / Published: 27 August 2016
View Full-Text   |   Download PDF [2243 KB, uploaded 27 August 2016]   |  

Abstract

Objective: To screen for influenza virus neuraminidase inhibition and to provide a reference for the clinical treatment of influenza using traditional Chinese medicines (TCM). In this study, 421 crude extracts (solubilized with petroleum ether, ethanol, ethyl acetate, and aqueous solvents) were obtained from 113 TCM. The medicine extracts were then reacted with oseltamivir, using 2’-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (MUNANA) as the substrate, to determine influenza virus neuraminidase activity using a standard fluorimetric assay. It was found that Chinese medicine extracts from Pyrola calliantha, Cynanchum wilfordii, Balanophora involucrata and Paeonia delavayi significantly inhibited neuraminidase activity at a concentration of 40 μg/mL. Dose-dependent inhibitory assays also revealed significant inhibition. The IC50 range of the TCM extracts for influenza virus neuraminidase was approximately 12.66–34.85 μg/mL, respectively. Some Chinese medicines have clear anti-influenza viral effects that may play an important role in the treatment of influenza through the inhibition of viral neuraminidase. The results of this study demonstrated that plant medicines can serve as a useful source of neuraminidase (NA) inhibitors and further investigation into the pharmacologic activities of these extracts is warranted. View Full-Text
Keywords: neuraminidase inhibition; screening; traditional Chinese medicine neuraminidase inhibition; screening; traditional Chinese medicine
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Yang, X.-Y.; Liu, A.-L.; Liu, S.-J.; Xu, X.-W.; Huang, L.-F. Screening for Neuraminidase Inhibitory Activity in Traditional Chinese Medicines Used to Treat Influenza. Molecules 2016, 21, 1138.

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