Efficient Enzyme-Free Biomimetic Sensors for Natural Phenol Detection
AbstractThe development of sensors and biosensors based on copper enzymes and/or copper oxides for phenol sensing is disclosed in this work. The electrochemical properties were studied by cyclic and differential pulse voltammetry using standard solutions of potassium ferrocyanide, phosphate/acetate buffers and representative natural phenols in a wide pH range (3.0 to 9.0). Among the natural phenols herein investigated, the highest sensitivity was observed for rutin, a powerful antioxidant widespread in functional foods and ubiquitous in the plant kingdom. The calibration curve for rutin performed at optimum pH (7.0) was linear in a broad concentration range, 1 to 120 µM (r = 0.99), showing detection limits of 0.4 µM. The optimized biomimetic sensor was also applied in total phenol determination in natural samples, exhibiting higher stability and sensitivity as well as distinct selectivity for antioxidant compounds. View Full-Text
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Ferreira Garcia, L.; Ribeiro Souza, A.; Sanz Lobón, G.; dos Santos, W.T.P.; Alecrim, M.F.; Fontes Santiago, M.; de Sotomayor, R.L.Á.; de Souza Gil, E. Efficient Enzyme-Free Biomimetic Sensors for Natural Phenol Detection. Molecules 2016, 21, 1060.
Ferreira Garcia L, Ribeiro Souza A, Sanz Lobón G, dos Santos WTP, Alecrim MF, Fontes Santiago M, de Sotomayor RLÁ, de Souza Gil E. Efficient Enzyme-Free Biomimetic Sensors for Natural Phenol Detection. Molecules. 2016; 21(8):1060.Chicago/Turabian Style
Ferreira Garcia, Luane; Ribeiro Souza, Aparecido; Sanz Lobón, Germán; dos Santos, Wallans T.P.; Alecrim, Morgana F.; Fontes Santiago, Mariângela; de Sotomayor, Rafael L.Á.; de Souza Gil, Eric. 2016. "Efficient Enzyme-Free Biomimetic Sensors for Natural Phenol Detection." Molecules 21, no. 8: 1060.
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