An Approach to Characterizing the Complicated Sequential Metabolism of Salidroside in Rats
Abstract
:1. Introduction
2. Results and Discussion
2.1. Stability of Salidroside in Artificial Gastric Juice and Intestinal Juice
2.2. Fragmentation of Salidroside Standard
2.3. Identification of Salidroside Metabolites in Different Plasma Samples
2.3.1. Parent Compound M0
2.3.2. Metabolite M1
2.3.3. Metabolite M2
2.3.4. Metabolite M3
2.3.5. Metabolite M4
2.4. The Sequential Process of Salidroside in the Digestive System
3. Experimental Section
3.1. Chemicals and Reagents
3.2. Preparation Perfusion Solution of Salidroside
3.3. Animals
3.4. Stability in Digestive Juice with Enzymes
3.5. Intestinal Flora Metabolism Method
3.6. Gut Wall Metabolism Method
3.7. Hepatic Metabolism Method
3.8. HPLC Analysis
3.9. LC/MS Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Metabolite | tR/min | Found (Da) | Calculated (Da) | Error (ppm) | Fragment ion m/z (Da) | Formula | Metabolite Description | Relative Percentage Area in Different Blood Samples 1 | |||
---|---|---|---|---|---|---|---|---|---|---|---|
GWM 2 | WOIF | WIIF | HM | ||||||||
M0 | 11.17 | 299.1126 | 299.1125 | 0.4 | 179.0556 | C14H20O7 | Parent | 94.26% | 99.59% | 43.85% | 22.93% |
119.0348 | |||||||||||
161.0451 | |||||||||||
143.0347 | |||||||||||
131.0346 | |||||||||||
113.0242 | |||||||||||
M1 | 8.32 | 313.0932 | 313.0918 | 4.5 | 175.0254 | C14H18O8 | Deglycosylation + Glucuronidation | 2.17% | 0.38% | 15.67% | 7.43% |
113.0249 | |||||||||||
295.0826 | |||||||||||
M2 | 8.93 | 475.1464 | 475.1446 | 3.8 | 457.1329 | C20H28O13 | Glucuronidation | 0.66% | 0.04% | 3.52% | 16.42% |
175.0242 | |||||||||||
299.1121 | |||||||||||
M3 | 10.80 | 379.0691 | 379.0693 | −0.6 | 217.0170 | C14H20O10S | Sulfation | 2.91% | ND | 7.00% | 11.00% |
299.1123 | |||||||||||
137.0605 | |||||||||||
119.0500 | |||||||||||
M4 | 11.61 | 217.0173 | 217.0165 | 3.4 | 137.0610 | C8H9O5S | Deglycosylation + Sulfation | ND 3 | ND | 29.96% | 42.23% |
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Luo, Z.; Ma, X.; Liu, Y.; Lu, L.; Yang, R.; Yu, G.; Sun, M.; Xin, S.; Tian, S.; Chen, X.; et al. An Approach to Characterizing the Complicated Sequential Metabolism of Salidroside in Rats. Molecules 2016, 21, 706. https://doi.org/10.3390/molecules21060706
Luo Z, Ma X, Liu Y, Lu L, Yang R, Yu G, Sun M, Xin S, Tian S, Chen X, et al. An Approach to Characterizing the Complicated Sequential Metabolism of Salidroside in Rats. Molecules. 2016; 21(6):706. https://doi.org/10.3390/molecules21060706
Chicago/Turabian StyleLuo, Zhiqiang, Xiaoyun Ma, Yang Liu, Lina Lu, Ruirui Yang, Guohua Yu, Mohan Sun, Shaokun Xin, Simin Tian, Xinjing Chen, and et al. 2016. "An Approach to Characterizing the Complicated Sequential Metabolism of Salidroside in Rats" Molecules 21, no. 6: 706. https://doi.org/10.3390/molecules21060706