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Molecules 2016, 21(6), 700; doi:10.3390/molecules21060700

Preparative Purification of Anti-Proliferative Diarylheptanoids from Betula platyphylla by High-Speed Counter-Current Chromatography

1
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 08826, Korea
2
Molecular Targets Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702-1202, USA
These authors contributed equally to this paper.
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas J. Schmidt
Received: 1 April 2016 / Revised: 20 May 2016 / Accepted: 24 May 2016 / Published: 28 May 2016
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [3914 KB, uploaded 28 May 2016]   |  

Abstract

A simple and rapid method using high-speed counter-current chromatography (HSCCC), along with bioassay-guided fractionation based on the anti-proliferative activity against renal and colon cancer cells, has been developed for the preparative separation of aceroside VIII (1) and platyphylloside (2) from Betula platyphylla. A solvent system composed of ethyl acetate/acetonitrile/water (1:0.1:1, v/v/v) was optimized for the separation. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase. Among these isolated diarylheptanoids, platyphylloside (2) showed anti-proliferative activity in the COLO205 and KM12 colon cells and renal cancer cell lines A498, U031, as well as in MG63 and MG 63.3 osteosarcoma cells. In addition, it showed dose dependent inhibitory effects in the NCI 60 cell line assay. These results suggest that the diarylheptanoids isolated from B. platyphylla with an efficient HSCCC method could be potential multi-targeted therapeutic agents for cancer. View Full-Text
Keywords: HSCCC; diarylheptanoid; anti-proliferation HSCCC; diarylheptanoid; anti-proliferation
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Cho, N.; Kim, H.W.; Kim, T.B.; Ransom, T.T.; Beutler, J.A.; Sung, S.H. Preparative Purification of Anti-Proliferative Diarylheptanoids from Betula platyphylla by High-Speed Counter-Current Chromatography. Molecules 2016, 21, 700.

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