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Molecules 2016, 21(5), 603; doi:10.3390/molecules21050603

High-Performance Liquid Chromatography with Diode Array Detector and Electrospray Ionization Ion Trap Time-of-Flight Tandem Mass Spectrometry to Evaluate Ginseng Roots and Rhizomes from Different Regions

State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking University, No. 38, Xueyuan Road, Haidian District, Beijing 100191, China
Institute of Special Wild Economic Animals and Plants Science, Chinese Academy of Agricultural Sciences, Changchun 130112, China
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China
Author to whom correspondence should be addressed.
Academic Editors: Vassilios Roussis and Efstathia Ioannou
Received: 27 February 2016 / Revised: 30 April 2016 / Accepted: 5 May 2016 / Published: 9 May 2016
(This article belongs to the Special Issue Triterpenes and Triterpenoids 2016)
View Full-Text   |   Download PDF [2704 KB, uploaded 9 May 2016]   |  


Ginseng, Panax ginseng C. A. Meyer, is an industrial crop in China and Korea. The functional components in ginseng roots and rhizomes are characteristic ginsenosides. This work developed a new high-performance liquid chromatography coupled with electrospray ionization ion trap time-of-flight multistage mass spectrometry (LC–ESI-IT-TOF-MSn) method to identify the triterpenoids. Sixty compounds (1–60) including 58 triterpenoids were identified from the ginseng cultivated in China. Substances 1, 2, 7, 15–20, 35, 39, 45–47, 49, 55–57, 59, and 60 were identified for the first time. To evaluate the quality of ginseng cultivated in Northeast China, this paper developed a practical liquid chromatography–diode array detection (LC–DAD) method to simultaneously quantify 14 interesting ginsenosides in ginseng collected from 66 different producing areas for the first time. The results showed the quality of ginseng roots and rhizomes from different sources was different due to growing environment, cultivation technology, and so on. The developed LC–ESI-IT-TOF-MSn method can be used to identify many more ginsenosides and the LC–DAD method can be used not only to assess the quality of ginseng, but also to optimize the cultivation conditions for the production of ginsenosides. View Full-Text
Keywords: Panax ginseng; ginsenosides; mass spectrometry; chemical substances Panax ginseng; ginsenosides; mass spectrometry; chemical substances

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Wang, H.-P.; Zhang, Y.-B.; Yang, X.-W.; Yang, X.-B.; Xu, W.; Xu, F.; Cai, S.-Q.; Wang, Y.-P.; Xu, Y.-H.; Zhang, L.-X. High-Performance Liquid Chromatography with Diode Array Detector and Electrospray Ionization Ion Trap Time-of-Flight Tandem Mass Spectrometry to Evaluate Ginseng Roots and Rhizomes from Different Regions. Molecules 2016, 21, 603.

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