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Molecules 2016, 21(5), 560; doi:10.3390/molecules21050560

De Novo Sequencing and Transcriptome Analysis of Pleurotus eryngii subsp. tuoliensis (Bailinggu) Mycelia in Response to Cold Stimulation

1
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China
2
Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99163, USA
3
Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China
4
China National GeneBank, Environmental Genomics, BGI, Shenzhen 518083, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 25 January 2016 / Revised: 21 April 2016 / Accepted: 21 April 2016 / Published: 17 May 2016
(This article belongs to the Section Molecular Diversity)
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Abstract

Cold stimulation of Bailinggu’s mycelia is the main factor that triggers primordia initiation for successful production of fruiting bodies under commercial cultivation. Yet, the molecular-level mechanisms involved in mycelia response to cold stimulation are still unclear. Here, we performed comparative transcriptomic analysis using RNA-Seq technology to better understand the gene expression regulation during different temporal stages of cold stimulation in Bailinggu. A total of 21,558 Bailinggu mycelia unigenes were de novo assembled and annotated from four libraries (control at 25 °C, plus cold stimulation treatments at −3 °C for a duration of 1–2 days, 5–6 days, and 9–10 days). GO and KEGG pathway analysis indicated that functional groups of differentially expressed unigenes associated with cell wall and membrane stabilization, calcium signaling and mitogen-activated protein kinases (MAPK) pathways, and soluble sugars and protein biosynthesis and metabolism pathways play a vital role in Bailinggu’s response to cold stimulation. Six hundred and seven potential EST-based SSRs loci were identified in these unigenes, and 100 EST-SSR primers were randomly selected for validation. The overall polymorphism rate was 92% by using 10 wild strains of Bailinggu. Therefore, these results can serve as a valuable resource for a better understanding of the molecular mechanisms associated with Bailinggu’s response to cold stimulation. View Full-Text
Keywords: Bailinggu; comparative transcriptomic analysis; cold stress; qPCR-PCR; EST-SSR Bailinggu; comparative transcriptomic analysis; cold stress; qPCR-PCR; EST-SSR
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Fu, Y.-P.; Liang, Y.; Dai, Y.-T.; Yang, C.-T.; Duan, M.-Z.; Zhang, Z.; Hu, S.-N.; Zhang, Z.-W.; Li, Y. De Novo Sequencing and Transcriptome Analysis of Pleurotus eryngii subsp. tuoliensis (Bailinggu) Mycelia in Response to Cold Stimulation. Molecules 2016, 21, 560.

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