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Molecules 2016, 21(4), 506; doi:10.3390/molecules21040506

Evaluation of Olive Fruit Lipoxygenase Extraction Protocols on 9- and 13-Z,E-HPODE Formation

Faculty of Science, Department of Chemistry, R. Boškovića 33, Split 21 000, Croatia
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 4 March 2016 / Revised: 13 April 2016 / Accepted: 13 April 2016 / Published: 20 April 2016
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [916 KB, uploaded 20 April 2016]   |  


In plant tissues, enzymes implicated in the lipoxygenase (LOX) pathway are responsible for the hydroperoxydation of polyunsaturated fatty acids, ultimately leading to the production of small chemical species involved in several physiological processes. During industrial olive oil production, these enzymes are activated upon crushing and grinding of olive fruit tissue, subsequently leading to the synthesis of volatile compounds responsible for the positive aroma and flavor of the oil. An investigation of LOX activity during olive fruit ripening and malaxation could assist in the production of oils with favorable aroma and taste. Therefore, a reliable method for olive LOX purification is crucial. Here we report a critical review of six LOX extraction protocols, two of which have shown minimum enzyme activity, possibly leading to misconceptions in the interpretation of experimental data. Future research concerning olive LOX should employ extraction methods that preserve enzyme activity. View Full-Text
Keywords: olive fruit; LOX; extraction protocols; enzyme activity; olive oil aroma olive fruit; LOX; extraction protocols; enzyme activity; olive oil aroma

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Soldo, B.; Šprung, M.; Mušac, G.; Pavela-Vrančić, M.; Ljubenkov, I. Evaluation of Olive Fruit Lipoxygenase Extraction Protocols on 9- and 13-Z,E-HPODE Formation. Molecules 2016, 21, 506.

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