Next Article in Journal
Aberrant Expression of Bacterial Pattern Recognition Receptor NOD2 of Basophils and Microbicidal Peptides in Atopic Dermatitis
Previous Article in Journal
New Insights into the Antibacterial Activity of Hydroxycoumarins against Ralstonia solanacearum
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle
Molecules 2016, 21(4), 470; doi:10.3390/molecules21040470

Identification of 3′,4′-Dimethoxy Flavonol-3-β-d-Glucopyranoside Metabolites in Rats by Liquid Chromatography-Electrospray Ionization Ion Trap Mass Spectrometry

1
Shanghai Institute of Technology, Shanghai 201418, China
2
Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
3
Center for New Drug Research, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
4
Shanghai 8 Plus 1 Pharmaceutical Technology Company Limited, Shanghai 200233, China
5
Department of Clinical Pharmacy, Shanghai General Hospital, School of medicine, Shanghai Jiao Tong University, No. 100 Haining Road, Shanghai 200080, China
6
Department of Pharmacy, Branch of Shanghai First People’s Hospital, Shanghai 200081, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 16 March 2016 / Revised: 1 April 2016 / Accepted: 6 April 2016 / Published: 9 April 2016
(This article belongs to the Section Metabolites)
View Full-Text   |   Download PDF [2022 KB, uploaded 9 April 2016]   |  

Abstract

A method using liquid chromatography-electrospray ionization ion trap mass spectrometry was established for the identification of metabolites in feces, urine and bile in rats after oral administration of 3′,4′-dimethoxy flavonol-3-β-d-glucopyranoside (abbreviated DF3G). Seven metabolites in rat feces, urine and bile were firstly identified on the basis of their MS fragmentation behaviors. Three metabolites were identified in the feces, 6 in the urine and 2 in the bile, which suggested that demethylation, deglycosylation and deglycosylation followed by glucuronide conjugation were the major metabolic pathways for DF3G in vivo. Hydrolyzation might be the first step in the absorption and metabolism of DF3G. The possible metabolic pathway was proposed for the first time. The established method was simple, reliable and sensitive, revealing that it could be used to rapidly screen and identify the structures of metabolites of DF3G to better understand its metabolism in vivo. View Full-Text
Keywords: LC-ESI/MSn; 3′,4′-dimethoxy flavonol-3-β-d-glucopyranoside; metabolites; metabolic pathway LC-ESI/MSn; 3′,4′-dimethoxy flavonol-3-β-d-glucopyranoside; metabolites; metabolic pathway
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zhu, Y.; Wen, J.; Cao, Y.; Jiang, Y.; Huang, J.; Fan, G.; Lou, Y. Identification of 3′,4′-Dimethoxy Flavonol-3-β-d-Glucopyranoside Metabolites in Rats by Liquid Chromatography-Electrospray Ionization Ion Trap Mass Spectrometry. Molecules 2016, 21, 470.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top