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Molecules 2016, 21(3), 123; doi:10.3390/molecules21030123

Novel Gold Nanoparticles Reduced by Sargassum glaucescens: Preparation, Characterization and Anticancer Activity

1
Innovation Center for Confectionery Technology (MANIS), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia
2
Darou Pakhsh Pharmaceutical Manufacturing Company, St 61th Daroupakhsh City, Km 18- Karaj Hiway, 1411816616 Tehran, Iran
3
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
4
College of Veterinary Medicine, University of Sulaimani, Street 11, Zone 207, Sulaimani Nwe, 00964 Sulaimani City, Kurdistan Region, Iraq
5
Malaysia-Japan International Institute of Technology, Universiti Technology Malaysia, Jalan Sultan Yahya Ahmad Petra, 54100 Kuala Lumpur, Malaysia
6
Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
7
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
8
Iranian Fisheries Research Organization, No. 297, West Fatemi Avenue, P. O. Box 14155-6116, 1411816616 Tehran, Iran
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Alexandru Mihai Grumezescu
Received: 24 September 2015 / Revised: 6 January 2016 / Accepted: 12 January 2016 / Published: 1 March 2016
(This article belongs to the Special Issue Pharmaceutical Nanotechnology: Novel Approaches)
View Full-Text   |   Download PDF [4052 KB, uploaded 1 March 2016]   |  

Abstract

The current study investigated the anticancer properties of gold nanoparticles (SG-stabilized AuNPs) synthesized using water extracts of the brown seaweed Sargassum glaucescens (SG). SG-stabilized AuNPs were characterized by ultraviolet-visible spectroscopy, transmission and scanning electron microscopy, and energy dispersive X-ray fluorescence spectrometry. The SG-stabilized AuNPs were stable and small at 3.65 ± 1.69 nm in size. The in vitro anticancer effect of SG-stabilized AuNPs was determined on cervical (HeLa), liver (HepG2), breast (MDA-MB-231) and leukemia (CEM-ss) cell lines using fluorescence microscopy, flow cytometry, caspase activity determination, and MTT assays. After 72 h treatment, SG-stabilized AuNPs was shown to be significant (p < 0.05) cytotoxic to the cancer cells in a dose- and time-dependent manner. The IC50 values of SG-stabilized AuNPs on the HeLa, HepG2, CEM-ss, MDA-MB-231 cell lines were 4.75 ± 1.23, 7.14 ± 1.45, 10.32 ± 1.5, and 11.82 ± 0.9 μg/mL, respectively. On the other hand, SG-stabilized AuNPs showed no cytotoxic effect towards the normal human mammary epithelial cells (MCF-10A). SG-stabilized AuNPs significantly (p < 0.05) arrest HeLa cell cycle at G2/M phase and significantly (p < 0.05) activated caspases-3 and -9 activities. The anticancer effect of SG-stabilized AuNPs is via the intrinsic apoptotic pathway. The study showed that SG-stabilized AuNPs is a good candidate to be developed into a chemotherapeutic compound for the treatment of cancers especially cervical cancer. View Full-Text
Keywords: Sargassum glaucescens; gold nanoparticles; anticancer properties; cervical cancer; in vitro study Sargassum glaucescens; gold nanoparticles; anticancer properties; cervical cancer; in vitro study
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MDPI and ACS Style

Ajdari, Z.; Rahman, H.; Shameli, K.; Abdullah, R.; Abd Ghani, M.; Yeap, S.; Abbasiliasi, S.; Ajdari, D.; Ariff, A. Novel Gold Nanoparticles Reduced by Sargassum glaucescens: Preparation, Characterization and Anticancer Activity. Molecules 2016, 21, 123.

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