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Molecules 2016, 21(11), 1567; doi:10.3390/molecules21111567

Pilot-Scale Production and Thermostability Improvement of the M23 Protease Pseudoalterin from the Deep Sea Bacterium Pseudoalteromonas sp. CF6-2

1
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China
2
Marine Biotechnology Research Center, Shandong University, Jinan 250100, China
3
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 8 October 2016 / Revised: 9 November 2016 / Accepted: 14 November 2016 / Published: 17 November 2016
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Abstract

Pseudoalterin is the most abundant protease secreted by the marine sedimental bacterium Pseudoalteromonas sp. CF6-2 and is a novel cold-adapted metalloprotease of the M23 family. Proteases of the M23 family have high activity towards peptidoglycan and elastin, suggesting their promising biomedical and biotechnological potentials. To lower the fermentive cost and improve the pseudoalterin production of CF6-2, we optimized the fermentation medium by using single factor experiments, added 0.5% sucrose as a carbon source, and lowered the usage of artery powder from 1.2% to 0.6%. In the optimized medium, pseudoalterin production reached 161.15 ± 3.08 U/mL, 61% greater than that before optimization. We further conducted a small-scale fermentation experiment in a 5-L fermenter and a pilot-scale fermentation experiment in a 50-L fermenter. Pseudoalterin production during pilot-scale fermentation reached 103.48 ± 8.64 U/mL, 77% greater than that before the medium was optimized. In addition, through single factor experiments and orthogonal tests, we developed a compound stabilizer for pseudoalterin, using medically safe sugars and polyols. This stabilizer showed a significant protective effect for pseudoalterin against enzymatic thermal denaturation. These results lay a solid foundation for the industrial production of pseudoalterin and the development of its biomedical and biotechnological potentials. View Full-Text
Keywords: marine bacteria; M23 proteases; fermentation; optimization; stability; cold-adapted enzymes marine bacteria; M23 proteases; fermentation; optimization; stability; cold-adapted enzymes
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Yang, J.; Yu, Y.; Tang, B.-L.; Zhong, S.; Shi, M.; Xie, B.-B.; Zhang, X.-Y.; Zhou, B.-C.; Zhang, Y.-Z.; Chen, X.-L. Pilot-Scale Production and Thermostability Improvement of the M23 Protease Pseudoalterin from the Deep Sea Bacterium Pseudoalteromonas sp. CF6-2. Molecules 2016, 21, 1567.

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