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Molecules 2016, 21(1), 76; doi:10.3390/molecules21010076

Antibacterial Activity of Alkaloid Fractions from Berberis microphylla G. Forst and Study of Synergism with Ampicillin and Cephalothin

1
Laboratorio de Productos Naturales, Instituto de la Patagonia, Universidad de Magallanes, Punta Arenas 6210427, Chile
2
Laboratorio de Química Ecológica, Departamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, Temuco 4811230, Chile
3
Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago 9170022, Chile
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 12 November 2015 / Revised: 26 December 2015 / Accepted: 6 January 2016 / Published: 11 January 2016
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Abstract

Berberis microphylla is a native plant that grows in Patagonia and is commonly used by aboriginal ethnic groups in traditional medicine as an antiseptic for different diseases. The present study evaluated the antibacterial and synergistic activity of alkaloid extracts of B. microphylla leaves, stems and roots used either individually or in combination with antibiotics against Gram-positive and Gram-negative bacteria. The in vitro antibacterial activities of leaf, stem and root alkaloid extracts had significant activity only against Gram-positive bacteria. Disc diffusion tests demonstrated that the root extract showed similar activity against B. cereus and S. epidermidis compared to commercial antibiotics, namely ampicillin and cephalothin, and pure berberine, the principal component of the alkaloid extracts, was found to be active only against S. aureus and S. epidermidis with similar activity to that of the root extract. The minimum inhibitory concentrations (MICs) of the alkaloid extracts ranged from 333 to 83 μg/mL, whereas minimum bactericidal concentrations (MBCs) varied from 717 to 167 μg/mL. In addition, synergistic or indifferent effects between the alkaloid extracts and antibiotics against bacterial strains were confirmed. View Full-Text
Keywords: Berberis microphylla; alkaloid extracts; antibacterial activity; synergism Berberis microphylla; alkaloid extracts; antibacterial activity; synergism
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Manosalva, L.; Mutis, A.; Urzúa, A.; Fajardo, V.; Quiroz, A. Antibacterial Activity of Alkaloid Fractions from Berberis microphylla G. Forst and Study of Synergism with Ampicillin and Cephalothin. Molecules 2016, 21, 76.

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