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Molecules 2016, 21(1), 40; doi:10.3390/molecules21010040

Application of Ultra-High-Performance Liquid Chromatography Coupled with LTQ-Orbitrap Mass Spectrometry for the Qualitative and Quantitative Analysis of Polygonum multiflorum Thumb. and Its Processed Products

Lab of Chinese Materia Medica Preparation, the Second College of Clinic Medicine, Guangzhou University of Chinese Medicine; Guangdong Province Institute of TCM, Guangzhou 510006, China
School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
Authors to whom correspondence should be addressed.
Academic Editor: Thomas J. Schmidt
Received: 13 July 2015 / Revised: 2 December 2015 / Accepted: 3 December 2015 / Published: 26 December 2015
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [1210 KB, uploaded 26 December 2015]   |  


In order to quickly and simultaneously obtain the chemical profiles and control the quality of the root of Polygonum multiflorum Thumb. and its processed form, a rapid qualitative and quantitative method, using ultra-high-performance liquid chromatography coupled with electrospray ionization-linear ion trap-Orbitrap hybrid mass spectrometry (UHPLC-LTQ-Orbitrap MSn) has been developed. The analysis was performed within 10 min on an AcQuity UPLC™ BEH C18 column with a gradient elution of 0.1% formic acid-acetonitrile at flow rate of 400 μL/min. According to the fragmentation mechanism and high resolution MSn data, a diagnostic ion searching strategy was used for rapid and tentative identification of main phenolic components and 23 compounds were simultaneously identified or tentatively characterized. The difference in chemical profiles between P. multiflorum and its processed preparation were observed by comparing the ions abundances of main constituents in the MS spectra and significant changes of eight metabolite biomarkers were detected in the P. multiflorum samples and their preparations. In addition, four of the representative phenols, namely gallic acid, trans-2,3,5,4′-tetra-hydroxystilbene-2-O-β-d-glucopyranoside, emodin and emodin-8-O-β-d-glucopyranoside were quantified by the validated UHPLC-MS/MS method. These phenols are considered to be major bioactive constituents in P. multiflorum, and are generally regarded as the index for quality assessment of this herb. The method was successfully used to quantify 10 batches of P. multiflorum and 10 batches of processed P. multiflorum. The results demonstrated that the method is simple, rapid, and suitable for the discrimination and quality control of this traditional Chinese herb. View Full-Text
Keywords: Polygonum multiflorum; LTQ-Orbitrap; qualitative and quantitative Polygonum multiflorum; LTQ-Orbitrap; qualitative and quantitative

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Wang, T.-H.; Zhang, J.; Qiu, X.-H.; Bai, J.-Q.; Gao, Y.-H.; Xu, W. Application of Ultra-High-Performance Liquid Chromatography Coupled with LTQ-Orbitrap Mass Spectrometry for the Qualitative and Quantitative Analysis of Polygonum multiflorum Thumb. and Its Processed Products. Molecules 2016, 21, 40.

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