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Molecules 2016, 21(1), 35; doi:10.3390/molecules21010035

A Herbal Formula, Atofreellage, Ameliorates Atopic Dermatitis-Like Skin Lesions in an NC/Nga Mouse Model

1
Liver and Immunology Research-Center, Daejeon Oriental Hospital of Daejeon University, 176-9, Daeheung-ro, Jung-Gu, Daejeon 34929, Korea
2
TKM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, Daejeon 34054, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 7 November 2015 / Revised: 18 December 2015 / Accepted: 21 December 2015 / Published: 25 December 2015
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [1984 KB, uploaded 25 December 2015]   |  

Abstract

We evaluated the anti-atopic dermatitis (AD) effect of Atofreellage (AF), a herbal formula composed of 10 medicinal plants. AD was induced on the dorsal skin areas of NC/Nga mice (male, seven weeks old) by daily application of 2,4-dinitrochlorobenzene (DNCB) for five weeks. After three weeks of DNCB application, 200 μL of AF (0, 25, 50 or 100 mg/mL) was applied to the skin lesions. Histological findings, blood cell populations, serum levels of immunoglobulin E (IgE), histamine, pro-inflammatory cytokines, and inflammatory signaling in the skin tissue, and T-helper cell type 2 (Th2)-related cytokines in splenocytes were analyzed. Histopathological findings showed AF treatment notably attenuated the thickness of dorsal skin, and eosinophil infiltration. AF treatment (especially 100 mg/mL) also demonstrably ameliorated the blood cell population abnormalities, as the notable elevation of serum concentrations of IgE, histamine, TNF-α, IL-6 and IL-1β were remarkably normalized by AF treatment. Western blot analysis evidenced the apparent normalization of inflammatory signals (ERK, p38 MAP kinase, JNK, and NF-κB) in the skin tissue. Additionally, AF treatment notably attenuated the activation of Th2-dominant cytokines (IL-13, IL-4, and IL-5) in Con A-treated splenocytes in an ex vivo assay. In conclusion, this study provides experimental evidence for the clinical relevance of Atofreellage. View Full-Text
Keywords: atopic dermatitis; herbal medicine; inflammation; NC/Nga murine model atopic dermatitis; herbal medicine; inflammation; NC/Nga murine model
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Kim, W.-Y.; Kim, H.-G.; Lee, H.-W.; Lee, J.-S.; Im, H.-J.; Kim, H.-S.; Lee, S.-B.; Son, C.-G. A Herbal Formula, Atofreellage, Ameliorates Atopic Dermatitis-Like Skin Lesions in an NC/Nga Mouse Model. Molecules 2016, 21, 35.

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