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Molecules 2016, 21(1), 126; doi:10.3390/molecules21010126

Serum Metabolomic Characterization of Liver Fibrosis in Rats and Anti-Fibrotic Effects of Yin-Chen-Hao-Tang

1
School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
2
E-Institute of Shanghai Municipal Education Committee, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
3
School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
4
Key Laboratory of Liver and Kidney Diseases (Ministry of Education), Institute of Liver Diseases, Shuguang Hospital, Shanghai 201203, China
5
Department of Chemistry, Tsinghua University, Beijing 100084, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 25 November 2015 / Revised: 31 December 2015 / Accepted: 14 January 2016 / Published: 21 January 2016
(This article belongs to the Section Metabolites)
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Abstract

Yin-Chen-Hao-Tang (YCHT) is a famous Chinese medicine formula which has long been used in clinical practice for treating various liver diseases, such as liver fibrosis. However, to date, the mechanism for its anti-fibrotic effects remains unclear. In this paper, an ultra-performance liquid chromatography-time-of-flight mass spectrometry (UPLC-TOF-MS)-based metabolomic study was performed to characterize dimethylnitrosamine (DMN)-induced liver fibrosis in rats and evaluate the therapeutic effects of YCHT. Partial least squares-discriminant analysis (PLS-DA) showed that the model group was well separated from the control group, whereas the YCHT-treated group exhibited a tendency to restore to the controls. Seven significantly changed fibrosis-related metabolites, including unsaturated fatty acids and lysophosphatidylcholines (Lyso-PCs), were identified. Moreover, statistical analysis demonstrated that YCHT treatment could reverse the levels of most metabolites close to the normal levels. These results, along with histological and biochemical examinations, indicate that YCHT has anti-fibrotic effects, which may be due to the suppression of oxidative stress and resulting lipid peroxidation involved in hepatic fibrogenesis. This study offers new opportunities to improve our understanding of liver fibrosis and the anti-fibrotic mechanisms of YCHT. View Full-Text
Keywords: serum metabolomics; liver fibrosis; Yin-Chen-Hao-Tang; anti-fibrotic effects; oxidative stress; lipid peroxidation; ultra-performance liquid chromatography-time-of-flight mass spectrometry; partial least squares-discriminant analysis serum metabolomics; liver fibrosis; Yin-Chen-Hao-Tang; anti-fibrotic effects; oxidative stress; lipid peroxidation; ultra-performance liquid chromatography-time-of-flight mass spectrometry; partial least squares-discriminant analysis
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Zhang, H.; Wang, X.; Hu, P.; Zhou, W.; Zhang, M.; Liu, J.; Wang, Y.; Liu, P.; Luo, G. Serum Metabolomic Characterization of Liver Fibrosis in Rats and Anti-Fibrotic Effects of Yin-Chen-Hao-Tang. Molecules 2016, 21, 126.

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