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Molecules 2015, 20(9), 16817-16832; doi:10.3390/molecules200916817

Optimized Extraction of Polysaccharides from Grateloupia livida (Harv.) Yamada and Biological Activities

1
Department of Pharmacology, Shantou University Medical College, Shantou 515041, China
2
Department of Pharmacy, First Affiliated Hospital, Shantou University Medical College, Shantou 515041, China
3
Traditional Chinese Medicine Laboratory, Shantou University Medical College, Shantou 515041, China
4
Department of Cardiovascular Diseases, First Affiliated Hospital, Shantou University Medical College, Shantou 515041, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 17 August 2015 / Revised: 7 September 2015 / Accepted: 10 September 2015 / Published: 16 September 2015
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [1974 KB, uploaded 16 September 2015]   |  

Abstract

Polysaccharides from Grateloupia livida (Harv.) Yamada (GL) were extracted by a heating circumfluence method. Single-factor experiments were performed for the three parameters: extraction time (X1), extraction temperature (X2) and the ratio of water to raw material (X3) and their test range. From preliminary experimental results, one type of the response surface methodology, the Box-Behnken design was applied for the optimizing polysaccharide extraction conditions. The experimental data obtained were fitted to a second-order polynomial equation. The optimal conditions were extraction time 5 h, extraction temperature 100 °C and ratio of water to raw material 70 mL/g. Under these conditions, the experimental yield was 39.22% ± 0.09%, which well matched the predicted value (39.25%), with 0.9774 coefficient of determination (R2). GL polysaccharides had scavenging activities for DPPH and hydroxyl radicals in vitro. The scavenging rates for both radicals peaked at 20 mg/mL GL concentration. However, the positive standard, VC (ascorbic acid), possessed stronger antioxidant activities than GL polysaccharides. Furthermore, the anticancer activity of GL polysaccharides on HepG2 cell proliferation increased dose- and time-dependently, but the positive standard, 5-fluorouracil (5-fu) showed more significant anticancer activity in this study. Overall, GL polysaccharides may have potential applications in the medical and food industries. View Full-Text
Keywords: Grateloupia livida (Harv.) Yamada; polysaccharide extraction; Box-Behnken design; antioxidant and anti-cancer activity Grateloupia livida (Harv.) Yamada; polysaccharide extraction; Box-Behnken design; antioxidant and anti-cancer activity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ye, D.; Jiang, Z.; Zheng, F.; Wang, H.; Zhang, Y.; Gao, F.; Chen, P.; Chen, Y.; Shi, G. Optimized Extraction of Polysaccharides from Grateloupia livida (Harv.) Yamada and Biological Activities. Molecules 2015, 20, 16817-16832.

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