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Molecules 2015, 20(9), 16643-16671; doi:10.3390/molecules200916643

Generation of Aptamers with an Expanded Chemical Repertoire

Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland
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Author to whom correspondence should be addressed.
Academic Editors: Alain O.A. Miller and Jean-Jacques Vanden Eynde
Received: 13 August 2015 / Revised: 28 August 2015 / Accepted: 1 September 2015 / Published: 14 September 2015
(This article belongs to the Special Issue Aptamers: Past, Present, and Future)
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Abstract

The enzymatic co-polymerization of modified nucleoside triphosphates (dN*TPs and N*TPs) is a versatile method for the expansion and exploration of expanded chemical space in SELEX and related combinatorial methods of in vitro selection. This strategy can be exploited to generate aptamers with improved or hitherto unknown properties. In this review, we discuss the nature of the functionalities appended to nucleoside triphosphates and their impact on selection experiments. The properties of the resulting modified aptamers will be described, particularly those integrated in the fields of biomolecular diagnostics, therapeutics, and in the expansion of genetic systems (XNAs). View Full-Text
Keywords: aptamers; modified nucleoside triphosphates; polymerases; SELEX; therapeutic oligonucleotides; chemically modified nucleic acids; synthetic genetic polymers aptamers; modified nucleoside triphosphates; polymerases; SELEX; therapeutic oligonucleotides; chemically modified nucleic acids; synthetic genetic polymers
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Diafa, S.; Hollenstein, M. Generation of Aptamers with an Expanded Chemical Repertoire. Molecules 2015, 20, 16643-16671.

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