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Molecules 2015, 20(8), 15147-15157; doi:10.3390/molecules200815147

Laccase Gene Expression and Vinasse Biodegradation by Trametes hirsuta Strain Bm-2

1
Laboratorio GeMBio, Centro de Investigación Científica de Yucatán A.C., Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97200, Yucatán, Mexico
2
Unidad de Energía Renovable, Centro de Investigación Científica de Yucatán A.C., Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97200, Yucatán, Mexico
3
Departamento de Ingeniería Química y Bioquímica, Instituto Tecnológico de Mérida, Av. Tecnológico Km 5, Mérida 97118, Yucatán, Mexico
*
Author to whom correspondence should be addressed.
Academic Editor: Anne S. Meyer
Received: 17 June 2015 / Revised: 11 August 2015 / Accepted: 13 August 2015 / Published: 19 August 2015
(This article belongs to the Special Issue Biocatalytic Lignin Modification)
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Abstract

Vinasse is the dark-colored wastewater that is generated by bioethanol distilleries from feedstock molasses. The vinasse that is generated from molasses contains high amounts of pollutants, including phenolic compounds and melanoindin. The goal of this work was to study the expression of laccase genes in the Trametes hirsuta strain Bm-2, isolated in Yucatan, Mexico, in the presence of phenolic compounds, as well as its effectiveness in removing colorants from vinasse. In the presence of all phenolic compounds tested (guaiacol, ferulic acid, and vanillic acid), increased levels of laccase-encoding mRNA were observed. Transcript levels in the presence of guaiacol were 40 times higher than those in the control. The lcc1 and lcc2 genes of T. hirsuta were differentially expressed; guaiacol and vanillin induced the expression of both genes, whereas ferulic acid only induced the expression of lcc2. The discoloration of vinasse was concomitant with the increase in laccase activity. The highest value of enzyme activity (2543.7 U/mL) was obtained in 10% (v/v) vinasse, which corresponded to a 69.2% increase in discoloration. This study demonstrates the potential of the Bm-2 strain of T. hirsuta for the biodegradation of vinasse. View Full-Text
Keywords: Trametes hirsuta; lacasses; gene expression; biodegradation; vinasse; phenolic compounds Trametes hirsuta; lacasses; gene expression; biodegradation; vinasse; phenolic compounds
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tapia-Tussell, R.; Pérez-Brito, D.; Torres-Calzada, C.; Cortés-Velázquez, A.; Alzate-Gaviria, L.; Chablé-Villacís, R.; Solís-Pereira, S. Laccase Gene Expression and Vinasse Biodegradation by Trametes hirsuta Strain Bm-2. Molecules 2015, 20, 15147-15157.

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