Qualitative and Quantitative Analysis of Major Triterpenoids in Alismatis Rhizoma by High Performance Liquid Chromatography/Diode-Array Detector/Quadrupole-Time-of-Flight Mass Spectrometry and Ultra-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry
Abstract
:1. Introduction
2. Results and Discussion
2.1. Optimization of Sample Preparation
2.2. Optimization of HPLC-DAD-Q-TOF MS Conditions
2.3. Optimization of UPLC-QqQ MS Conditions
Peak Number | Analytes | Quantification Transition (m/z) | Cone Voltage (V) | Collision Energy (ce) |
---|---|---|---|---|
1 | 16-oxo-Alisol A-23acetate | 529.34→451.34 | 30 | 18 |
2 | 16-oxo-Alisol A-24acetate | 529.34→451.34 | 30 | 18 |
3 | Alisol C | 487.33→415.35 | 40 | 18 |
4 | Alisol F | 471.33→339.33 | 35 | 12 |
5 | Alisol C-23acetate | 529.32→451.32 | 40 | 21 |
6 | Alisol L | 469.31→397.31 | 35 | 22 |
7 | Alisol F 24-acetate | 513.31→339.33 | 35 | 12 |
8 | Alisol A | 473.39→383.39 | 40 | 11 |
9 | Alisol A 23-acetate | 497.36→365.36 | 40 | 16 |
10 | Alisol A 24-acetate | 497.36→365.36 | 40 | 15 |
IS | Glycyrrhetinic acid (IS) | 471.32→317.28 | 40 | 28 |
11 | Alisol G | 455.31→437.31 | 35 | 10 |
12 | Alisol B | 455.31→437.31 | 40 | 11 |
13 | Alisol B 23-acetate | 497.29→437.29 | 40 | 11 |
14 | 11-deoxy-Alisol B | 479.31→479.31 | 45 | 10 |
2.4. Identification of Compounds with HPLC-DAD-Q-TOF MS
Peak Number | tR (min) | MS1 | Error (ppm) | Fragment Ions Collected in Positive Mode | Molecular Formula | λmax (nm) | Identity |
---|---|---|---|---|---|---|---|
1 | 11.81 | 505.3534 [M + H]+ | 1.0 | 487.3421 [M + H − H2O]+, 469.3317 [M + H − 2H2O]+, 451.3416 [M + H − 3H2O]+, 415.2845 [M + H − C4H10O2]+, 397.2745 [M + H − C4H10O2 − H2O]+ | C 30H48O6 | 245 | 16-oxo-Alisol A a |
2 | 13.19 | 547.3638 [M + H]+ | 0.5 | 529.3528 [M + H − H2O]+, 511.3421 [M + H − 2H2O]+, 487.3422 [M + H − HAc]+, 469.3316 [M + H − Hac − H2O]+, 415.2848 [M + H − C6H12O3]+, 397.2745 [M + H − C6H12O3 − H2O]+ | C 32H50O7 | 245 | 16-oxo-Alisol A 23-actetate a |
3 | 15.63 | 547.3624 [M + H]+ | −2.0 | 529.3529 [M + H − H2O]+, 511.3429 [M + H − 2H2O]+, 487.3423 [M + H − HAc]+, 469.3318 [M + H – Hac − H2O]+, 415.2847 [M + H − C6H12O3]+, 397.2744 [M + H − C6H12O3 − H2O]+ | C 32H50O7 | 245 | 16-oxo-Alisol A 24-actetate a |
4 | 16.70 | 507.3687 [M + H]+ | 0.2 | 489.3577 [M + H − H2O]+, 471.3471 [M + H − 2H2O]+, 453.3365 [M + H − 3H2O]+, 417.3007 [M + H − C4H10O2]+, 399.2896 [M + H − C4H10O2 − H2O]+ | C 30H50O6 | - | 13,17-epoxy-Alisol A a |
5 | 17.31 | 487.3423 [M + H]+ | −0.2 | 469.3314 [M + H − H2O]+, 451.3213 [M + H − 2H2O]+, 415.2650 [M + H − C4H8O]+, 397.2741 [M + H − H2O − C4H8O]+, 379.3427 [M + H − 2H2O − C4H8O]+ | C 30H46O5 | 245 | Alisol C a |
6 | 19.36 | 487.3429 [M + H]+ | 1.0 | 469.3319 [M + H − H2O]+, 451.3215 [M + H − 2H2O]+, 397.2742 [M+H-C4H10O2]+ | C 30H46O5 | 287 | 16-oxo-11-anhydro-Alisol A a |
7 | 19.72 | 489.3562 [M + H]+ | −2.7 | 471.3471 [M + H − H2O]+, 453.3367 [M + H − 2H2O]+, 399.2896 [M+H-C4H10O2]+ | C 30H48O5 | 245 | 16-oxo-11-deoxy-Alisol A |
8 | 21.46 | 549.3798 [M + H]+ | 1.3 | 531.3685 [M + H − H2O]+, 513.3579 [M + H − 2H2O]+, 489.3585 [M + H − HAc]+, 471.3470 [M + H − Hac − H2O]+, 417.3005 [M + H − C6H12O3]+, 399.2896 [M + H − C6H12O3 − H2O]+ | C 32H52O7 | - | 13,17-epoxy-Alisol A 24-acetate a |
9 | 23.30 | 471.3464 [M + H]+ | −2.1 | 453.3367 [M + H − H2O]+, 381.2791 [M + H − C4H8O − H2O]+, 339.2684 [M + H − C4H8O − H2O − C2H2O]+ | C 30H46O4 | - | 16,23-oxido-Alisol B |
10 | 23.37 | 489.3583 [M + H]+ | 0.6 | 471.3472 [M + H − H2O]+, 453.3366 [M + H − 2H2O]+, 381.2798 [M + H − H2O − C4H10O2]+, 339.2687 [M + H − H2O − C4H10O2 − C2H2O]+ | C 30H48O5 | - | Alisol F a |
11 | 24.33 | 529.3522 [M + H]+ | −1.3 | 511.3424 [M + H − H2O]+, 469.3313 [M + H − HAc]+, 451.3212 [M + H − HAc − H2O]+, 415.2652 [M + H − C6H10O2]+,397.2741 [M + H − C6H10O2 − H2O]+ | C 32H48O6 | 245 | Alisol C 23-acetate a |
12 | 24.75 | 489.3586 [M + H]+ | 1.2 | 471.3477 [M + H − H2O]+, 453.3363 [M + H − 2H2O]+, 417.3006 [M + H − C4H8O]+, 399.2895 [M + H − C4H8O − H2O]+ | C 30H48O5 | - | 13,17-epoxy-Alisol B a |
13 | 25.46 | 471.3482 [M + H]+ | 1.7 | 453.3368 [M + H − H2O]+, 399.2894 [M + H − C4H8O]+ | C 30H46O4 | 245 | 11-deoxy-Alisol C |
14 | 25.91 | 469.3315 [M + H]+ | −0.6 | 451.3216 [M + H − H2O]+, 397.2745 [M + H − C4H8O]+ | C 30H44O4 | 287 | Alisol L a |
15 | 27.00 | 531.3694 [M + H]+ | 1.5 | 513.3585 [M + H − H2O]+, 495.3598 [M + H − 2H2O]+, 453.3367 [M + H − H2O − HAc]+, 435.3592 [M + H − 2H2O − HAc]+, 381.2791 [M + H − H2O − C6H12O3]+, 339.2684 [M + H − H2O − C6H12O3 − C2H2O]+ | C 32H50O6 | - | Alisol F 24-actetate a |
16 | 27.75 | 491.3731 [M + H]+ | 0.0 | 473.3630 [M + H − H2O]+, 455.3524 [M + H − 2H2O]+, 437.3418 [M + H − 3H2O]+, 383.2984 [M + H − H2O − C4H10O2]+ | C 30H50O5 | - | Alisol A a |
17 | 29.07 | 533.3773 [M + H]+ | 0.4 | 515.3735 [M + H − H2O]+, 497.3633 [M + H − 2H2O]+, 455.3526 [M + H − HAc − H2O] +, 437.3422 [M + H − HAc − 2H2O]+, 383.2983 [M + H − C6H12O3 − H2O]+ | C 32H52O6 | - | Alisol A 23-acetate a |
18 | 33.90 | 533.3848 [M + H]+ | 1.1 | 515.3736 [M + H − H2O]+, 497.3634 [M + H − 2H2O]+, 455.3528 [M + H − HAc − H2O] +, 437.3420 [M + H − HAc − 2H2O]+, 383.2981 [M + H − C6H12O3 − H2O]+ | C 32H52O6 | - | Alisol A 24-acetate a |
19 | 34.95 | 531.3685 [M + H]+ | −0.2 | 513.3581 [M + H − H2O]+, 495.3476 [M + H − 2H2O]+, 471.3478 [M + H − HAc]+, 453.3367 [M + H − HAc − H2O]+, 417.3008 [M + H − C6H10O2]+, 399.2895 [M + H − C6H10O2 − H2O]+ | C 32H50O6 | - | 13,17-epoxy-Alisol B 23-acetate a |
20 | 35.87 | 511.3418 [M + H]+ | −1.2 | 451.3321 [M + H − HAc]+, 397.2743 [M + H − C6H10O2]+ | C 32H46O5 | 287 | Alisol L 23-acetate a |
21 | 38.87 | 473.3638 [M + H]+ | 1.5 | 455.3527 [M + H − H2O]+, 437.3425 [M + H − 2H2O]+, 383.2981 [M + H − H2O − C4H8O]+ | C 30H48O4 | - | Alisol G a |
22 | 40.71 | 473.3639 [M + H]+ | 1.7 | 455.3523 [M + H − H2O]+, 437.2426 [M + H − 2H2O]+, 383.2982 [M + H − H2O − C4H8O]+ | C 30H48O4 | - | Alisol B a |
23 | 49.06 | 515.3739 [M + H]+ | 0.4 | 497.3631 [M + H − H2O]+, 479.3519 [M + H − 2H2O]+, 437.3431 [M + H − H2O − HAc]+, 383.2983 [M + H − H2O − C6H10O2]+ | C 32H50O5 | - | Alisol B 23-acetate a |
24 | 54.55 | 457.3675 [M + H]+ | −1.5 | 439.3574 [M + H − H2O]+, 385.3102 [M + H − C4H8O]+ | C 30H48O3 | - | 11-deoxy-Alisol B a |
25 | 61.12 | 499.3794 [M + H]+ | 1.4 | 439.3578 [M + H − HAc]+, 385.3105 [M + H − C6H10O2]+ | C 32H50O4 | - | 11-deoxy-Alisol B 23-acetate |
2.5. Method Validation
Analytes | tR (min) | Calibration Curve | Linear Range μg/mL | r (n = 6) | LOD ng/mL | LOQ ng/mL |
---|---|---|---|---|---|---|
16-oxo-Alisol A 23-acetate | 1.48 | Y = 15.87X − 0.014 | 0.008–0.103 | 0.9999 | 1.17 | 3.91 |
16-oxo-Alisol A 24-acetate | 1.71 | Y = 17.21X − 0.002 | 0.009–0.138 | 0.9990 | 1.82 | 6.05 |
Alisol C | 1.95 | Y = 5.005X − 0.043 | 0.037–1.490 | 0.9980 | 3.63 | 12.1 |
Alisol F | 2.78 | Y = 2.496X − 0.013 | 0.062–1.125 | 0.9989 | 2.45 | 8.17 |
Alisol C 23-acetate | 2.87 | Y = 9.118X − 0.029 | 0.045–1.352 | 0.9990 | 1.70 | 5.67 |
Alisol L | 3.10 | Y = 12.22X − 0.054 | 0.024–0.652 | 0.9996 | 1.68 | 5.59 |
Alisol F 24-acetate | 3.25 | Y = 2.160X − 0.038 | 0.078–1.060 | 0.9990 | 7.01 | 21.0 |
Alisol A | 3.38 | Y = 3.125X − 0.016 | 0.066–3.333 | 0.9982 | 1.61 | 5.35 |
Alisol A 23-acetate | 3.54 | Y = 3.441X − 0.017 | 0.042–1.080 | 0.9993 | 1.73 | 5.75 |
Alisol A 24-acetate | 4.05 | Y = 3.505X − 0.010 | 0.049–3.240 | 0.9990 | 1.01 | 4.37 |
Alisol G | 4.61 | Y = 6.788X − 0.019 | 0.026–0.525 | 0.9980 | 3.63 | 12.1 |
Alisol B | 4.84 | Y = 1.635X − 0.013 | 0.197–5.444 | 0.9982 | 3.40 | 10.6 |
Alisol B 23-acetate | 5.67 | Y = 2.678X − 0.023 | 0.294–9.790 | 0.9993 | 3.04 | 10.1 |
11-deoxy-Alisol B | 6.36 | Y = 2.584X − 0.058 | 0.089–1.740 | 0.9990 | 9.23 | 27.4 |
Analytes | Precision for Standard Solution (n = 6) | Repeatability from Real Samples | Stability (%) | Accuracy | |||
---|---|---|---|---|---|---|---|
Inter-Day RSD (%) | Intra-Day RSD (%) | RSD (%, n = 6) | Mean (mg/g) | Recovery (%) | RSD (%) | ||
16-oxo-Alisol A 23-acetate | 3.14 | 2.73 | 3.17 | 0.011 | 97.54 ± 2.49 | 100.2 | 3.10 |
16-oxo-Alisol A 24-acetate | 3.52 | 3.55 | 3.17 | 0.006 | 99.23 ± 2.39 | 101.7 | 2.02 |
Alisol C | 3.94 | 3.79 | 3.97 | 0.128 | 98.57 ± 3.72 | 99.62 | 2.44 |
Alisol F | 1.53 | 1.46 | 2.21 | 0.182 | 101.23 ± 2.73 | 103.8 | 2.53 |
Alisol C 23-acetate | 2.14 | 3.60 | 3.02 | 0.174 | 96.22 ± 3.82 | 98.11 | 2.24 |
Alisol L | 3.96 | 2.29 | 2.47 | 0.012 | 98.93 ± 1.59 | 101.3 | 4.06 |
Alisol F 24-acetate | 2.22 | 1.28 | 2.86 | 0.089 | 99.75 ± 1.98 | 100.8 | 2.21 |
Alisol A | 2.15 | 1.82 | 3.49 | 0.595 | 100.24 ± 3.52 | 98.17 | 2.15 |
Alisol A 23-acetate | 2.14 | 1.81 | 4.03 | 0.105 | 96.88 ± 3.59 | 103.8 | 3.14 |
Alisol A 24-acetate | 2.85 | 2.23 | 3.96 | 0.110 | 97.59 ± 3.61 | 102.0 | 3.84 |
Alisol G | 2.08 | 3.22 | 3.01 | 0.031 | 97.92 ± 2.02 | 99.87 | 2.58 |
Alisol B | 3.70 | 3.00 | 4.25 | 1.086 | 98.36 ± 1.30 | 99.34 | 3.20 |
Alisol B 23-acetate | 2.22 | 1.48 | 2.31 | 1.465 | 100.56 ± 1.61 | 101.0 | 2.82 |
11-deoxy-Alisol B | 2.04 | 1.18 | 2.22 | 0.104 | 99.37 ± 3.19 | 101.2 | 3.04 |
2.6. Sample Analysis
NO. | Sample | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
S1 | Fujian 1 | 0.009 | 0.008 | 0.055 | 0.041 | 0.121 | 0.012 | 0.082 | 0.067 | 0.029 | 0.052 | 0.020 | 0.307 | 1.420 | 0.180 |
S2 | Fujian 2 | 0.006 | 0.007 | 0.068 | 0.044 | 0.113 | 0.013 | 0.081 | 0.069 | 0.032 | 0.061 | 0.020 | 0.325 | 1.546 | 0.177 |
S3 | Fujian 3 | 0.006 | 0.007 | 0.027 | 0.063 | 0.118 | 0.013 | ND | 0.075 | 0.030 | 0.069 | 0.024 | 0.118 | 1.560 | 0.183 |
S4 | Fujian 4 | 0.006 | 0.008 | 0.025 | 0.047 | 0.219 | 0.018 | 0.08 | 0.070 | 0.021 | 0.028 | 0.020 | 0.324 | 1.216 | 0.124 |
S5 | Fujian 5 | 0.005 | 0.005 | 0.023 | 0.046 | 0.204 | 0.018 | 0.081 | 0.081 | 0.023 | 0.028 | 0.021 | 0.291 | 1.780 | 0.122 |
S6 | Fujian 6 | 0.006 | 0.007 | 0.027 | 0.049 | 0.244 | 0.020 | 0.085 | 0.075 | 0.024 | 0.030 | 0.025 | 0.342 | 1.502 | 0.131 |
S7 | Fujian 7 | 0.007 | 0.008 | 0.070 | 0.057 | 0.141 | 0.014 | ND | 0.079 | 0.031 | 0.061 | 0.023 | 0.246 | 1.459 | 0.127 |
S8 | Fujian 8 | 0.006 | 0.009 | 0.045 | 0.048 | 0.153 | 0.013 | 0.081 | 0.080 | 0.032 | 0.062 | 0.021 | 0.237 | 1.451 | 0.095 |
S9 | Fujian 9 | 0.006 | 0.006 | 0.022 | 0.042 | 0.113 | 0.014 | 0.082 | 0.069 | 0.047 | 0.053 | 0.027 | 0.104 | 1.273 | 0.133 |
S10 | Fujian 10 | 0.005 | 0.007 | 0.024 | 0.048 | 0.099 | 0.018 | 0.083 | 0.082 | 0.024 | 0.029 | 0.022 | 0.333 | 1.769 | 0.121 |
S11 | Fujian 11 | 0.008 | 0.008 | 0.054 | 0.042 | 0.121 | 0.013 | 0.082 | 0.068 | 0.029 | 0.051 | 0.020 | 0.308 | 1.420 | 0.181 |
S12 | Fujian 12 | 0.007 | 0.007 | 0.067 | 0.044 | 0.114 | 0.013 | 0.081 | 0.069 | 0.032 | 0.061 | 0.020 | 0.326 | 1.546 | 0.178 |
S13 | Fujian 13 | 0.006 | 0.007 | 0.027 | 0.063 | 0.118 | 0.013 | ND | 0.075 | 0.030 | 0.069 | 0.024 | 0.118 | 1.560 | 0.183 |
S14 | Fujian 14 | 0.005 | 0.008 | 0.026 | 0.048 | 0.219 | 0.019 | 0.08 | 0.070 | 0.021 | 0.029 | 0.021 | 0.324 | 1.216 | 0.124 |
S15 | Fujian 15 | 0.006 | 0.005 | 0.024 | 0.046 | 0.205 | 0.018 | 0.082 | 0.081 | 0.024 | 0.028 | 0.021 | 0.291 | 1.780 | 0.122 |
S16 | Fujian 16 | 0.006 | 0.007 | 0.027 | 0.049 | 0.244 | 0.020 | 0.085 | 0.076 | 0.024 | 0.030 | 0.025 | 0.343 | 1.300 | 0.132 |
S17 | Fujian 17 | 0.007 | 0.008 | 0.070 | 0.057 | 0.141 | 0.014 | ND | 0.079 | 0.031 | 0.061 | 0.024 | 0.246 | 1.459 | 0.127 |
S18 | Fujian 18 | 0.007 | 0.009 | 0.045 | 0.048 | 0.153 | 0.013 | 0.081 | 0.080 | 0.032 | 0.062 | 0.021 | 0.238 | 1.452 | 0.095 |
S19 | Fujian 19 | 0.006 | 0.006 | 0.022 | 0.043 | 0.114 | 0.014 | 0.083 | 0.069 | 0.047 | 0.054 | 0.027 | 0.104 | 1.273 | 0.133 |
S20 | Fujian 20 | 0.005 | 0.007 | 0.024 | 0.048 | 0.099 | 0.018 | 0.083 | 0.082 | 0.024 | 0.029 | 0.022 | 0.333 | 1.569 | 0.122 |
S21 | Fujian 21 | 0.007 | 0.008 | 0.044 | 0.049 | 0.153 | 0.013 | 0.082 | 0.080 | 0.032 | 0.062 | 0.022 | 0.237 | 1.451 | 0.095 |
S22 | Sichuan 1 | 0.011 | 0.008 | 0.207 | 0.184 | 0.202 | 0.013 | 0.085 | 0.802 | 0.115 | 0.543 | 0.038 | 0.905 | 1.418 | 0.163 |
S23 | Sichuan 2 | 0.010 | 0.007 | 0.145 | 0.184 | 0.138 | 0.013 | 0.079 | 0.594 | 0.111 | 0.803 | 0.035 | 1.208 | 2.002 | 0.138 |
S24 | Sichuan 3 | 0.009 | 0.007 | 0.137 | 0.213 | 0.131 | 0.013 | 0.079 | 0.568 | 0.105 | 0.807 | 0.032 | 1.232 | 2.032 | 0.166 |
S25 | Sichuan 4 | 0.011 | 0.006 | 0.128 | 0.182 | 0.174 | 0.012 | 0.089 | 0.595 | 0.105 | 0.110 | 0.031 | 1.086 | 1.465 | 0.104 |
S26 | Sichuan 5 | 0.008 | 0.006 | 0.108 | 0.131 | 0.065 | 0.017 | ND | 0.594 | 0.035 | 0.102 | 0.022 | 0.784 | 1.438 | 0.109 |
S27 | Sichuan 6 | 0.010 | 0.008 | 0.077 | 0.156 | 0.069 | 0.013 | 0.082 | 0.567 | 0.102 | 0.279 | 0.025 | 0.740 | 1.163 | 0.104 |
S28 | Sichuan 7 | 0.012 | 0.009 | 0.034 | 0.211 | 0.143 | 0.012 | ND | 0.756 | 0.107 | 0.271 | 0.026 | 1.016 | 1.217 | 0.061 |
S29 | Sichuan 8 | 0.015 | 0.011 | 0.069 | 0.221 | 0.241 | 0.013 | 0.081 | 0.707 | 0.114 | 0.297 | 0.029 | 0.525 | 1.513 | 0.123 |
S30 | Sichuan 9 | 0.012 | 0.007 | 0.104 | 0.118 | 0.114 | 0.016 | ND | 0.445 | 0.047 | 0.104 | 0.024 | 0.663 | 1.821 | 0.052 |
S31 | Sichuan 10 | 0.012 | 0.010 | 0.073 | 0.109 | 0.182 | 0.015 | 0.090 | 0.491 | 0.062 | 0.155 | 0.021 | 0.912 | 1.131 | 0.072 |
S32 | Sichuan 11 | 0.010 | 0.009 | 0.206 | 0.184 | 0.202 | 0.014 | 0.085 | 0.802 | 0.114 | 0.544 | 0.038 | 0.906 | 1.419 | 0.164 |
S33 | Sichuan 12 | 0.011 | 0.010 | 0.145 | 0.185 | 0.138 | 0.013 | 0.079 | 0.594 | 0.111 | 0.803 | 0.035 | 1.208 | 2.001 | 0.139 |
S34 | Sichuan 13 | 0.009 | 0.008 | 0.138 | 0.213 | 0.131 | 0.014 | 0.081 | 0.568 | 0.105 | 0.807 | 0.032 | 1.231 | 2.032 | 0.166 |
S35 | Sichuan 14 | 0.011 | 0.010 | 0.128 | 0.183 | 0.174 | 0.012 | 0.089 | 0.596 | 0.105 | 0.111 | 0.031 | 1.086 | 1.465 | 0.104 |
S36 | Sichuan 15 | 0.009 | 0.007 | 0.108 | 0.131 | 0.065 | 0.017 | ND | 0.594 | 0.035 | 0.102 | 0.023 | 0.784 | 1.439 | 0.109 |
S37 | Sichuan 16 | 0.010 | 0.008 | 0.078 | 0.156 | 0.069 | 0.014 | 0.082 | 0.567 | 0.103 | 0.279 | 0.025 | 0.741 | 1.663 | 0.105 |
S38 | Sichuan 17 | 0.011 | 0.009 | 0.034 | 0.212 | 0.144 | 0.012 | ND | 0.757 | 0.107 | 0.271 | 0.026 | 1.016 | 1.217 | 0.061 |
S39 | Sichuan 18 | 0.015 | 0.011 | 0.069 | 0.221 | 0.241 | 0.013 | 0.081 | 0.707 | 0.114 | 0.297 | 0.029 | 0.525 | 1.513 | 0.123 |
S40 | Sichuan 19 | 0.012 | 0.007 | 0.105 | 0.118 | 0.114 | 0.016 | ND | 0.446 | 0.048 | 0.105 | 0.024 | 0.663 | 1.522 | 0.053 |
S41 | Sichuan 20 | 0.013 | 0.011 | 0.073 | 0.109 | 0.183 | 0.015 | 0.091 | 0.491 | 0.062 | 0.155 | 0.021 | 0.913 | 1.131 | 0.072 |
S42 | Sichuan 21 | 0.008 | 0.007 | 0.109 | 0.132 | 0.065 | 0.017 | ND | 0.595 | 0.036 | 0.103 | 0.023 | 0.784 | 1.438 | 0.109 |
S43 | Sichuan 22 | 0.010 | 0.008 | 0.077 | 0.156 | 0.069 | 0.014 | 0.082 | 0.567 | 0.102 | 0.279 | 0.025 | 0.741 | 1.164 | 0.105 |
3. Experimental Section
3.1. Materials and Standards, Reagents
3.2. Preparation of Standard Solution and Samples
3.3. Qualitative Chromatographic Conditions
3.4. Quantitative Chromatographic Conditions
3.5. Validation of the Quantitative Method
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Zhao, W.; Huang, X.; Li, X.; Zhang, F.; Chen, S.; Ye, M.; Huang, M.; Xu, W.; Wu, S. Qualitative and Quantitative Analysis of Major Triterpenoids in Alismatis Rhizoma by High Performance Liquid Chromatography/Diode-Array Detector/Quadrupole-Time-of-Flight Mass Spectrometry and Ultra-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry. Molecules 2015, 20, 13958-13981. https://doi.org/10.3390/molecules200813958
Zhao W, Huang X, Li X, Zhang F, Chen S, Ye M, Huang M, Xu W, Wu S. Qualitative and Quantitative Analysis of Major Triterpenoids in Alismatis Rhizoma by High Performance Liquid Chromatography/Diode-Array Detector/Quadrupole-Time-of-Flight Mass Spectrometry and Ultra-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry. Molecules. 2015; 20(8):13958-13981. https://doi.org/10.3390/molecules200813958
Chicago/Turabian StyleZhao, Wanli, Xiaoqiang Huang, Xiaoyan Li, Fangfang Zhang, Sainan Chen, Miao Ye, Mingqing Huang, Wen Xu, and Shuisheng Wu. 2015. "Qualitative and Quantitative Analysis of Major Triterpenoids in Alismatis Rhizoma by High Performance Liquid Chromatography/Diode-Array Detector/Quadrupole-Time-of-Flight Mass Spectrometry and Ultra-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry" Molecules 20, no. 8: 13958-13981. https://doi.org/10.3390/molecules200813958