Separation of Polyphenols and Caffeine from the Acetone Extract of Fermented Tea Leaves (Camellia sinensis) Using High-Performance Countercurrent Chromatography
Abstract
:1. Introduction
2. Results and Discussion
2.1. HPLC-PDA Analysis of Sample Extract
2.2. Evaluation of Partition Coefficient (K) Value
HEMWat System (v/v) | Partition Coefficient (K) Value | ||||||
---|---|---|---|---|---|---|---|
1 (K1) | α1 | 2 (K2) | α2 | 3 (K3) | α3 | 4 (K4) | |
3:7:3:7 | 0.27 | - | 0.03 | 2.33 | 0.07 | 2.14 | 0.15 |
2:8:2:8 | 0.39 | - | 0.23 | 2.04 | 0.47 | 1.74 | 0.82 |
1:9:1:9 | 0.60 | 2.28 | 1.37 | 1.78 | 2.45 | 1.52 | 3.74 |
2.3. HPCCC Separation of Sample Extract
3. Experimental Section
3.1. General Experimental Procedures
3.2. Preparation of Sample Extract
3.3. HPLC-PDA Analysis
3.4. Evaluation of Partition Coefficient Value
3.5. HPCCC Procedure
3.6. Identification of HPCCC Peak Fractions
Acknowledgements
Author Contributions
Conflicts of Interest
References
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Choi, S.J.; Hong, Y.D.; Lee, B.; Park, J.S.; Jeong, H.W.; Kim, W.G.; Shin, S.S.; Yoon, K.D. Separation of Polyphenols and Caffeine from the Acetone Extract of Fermented Tea Leaves (Camellia sinensis) Using High-Performance Countercurrent Chromatography. Molecules 2015, 20, 13216-13225. https://doi.org/10.3390/molecules200713216
Choi SJ, Hong YD, Lee B, Park JS, Jeong HW, Kim WG, Shin SS, Yoon KD. Separation of Polyphenols and Caffeine from the Acetone Extract of Fermented Tea Leaves (Camellia sinensis) Using High-Performance Countercurrent Chromatography. Molecules. 2015; 20(7):13216-13225. https://doi.org/10.3390/molecules200713216
Chicago/Turabian StyleChoi, Soo Jung, Yong Deog Hong, Bumjin Lee, Jun Seong Park, Hyun Woo Jeong, Wan Gi Kim, Song Seok Shin, and Kee Dong Yoon. 2015. "Separation of Polyphenols and Caffeine from the Acetone Extract of Fermented Tea Leaves (Camellia sinensis) Using High-Performance Countercurrent Chromatography" Molecules 20, no. 7: 13216-13225. https://doi.org/10.3390/molecules200713216