Next Article in Journal
Identification and Expression Analysis of Glucosinolate Biosynthetic Genes and Estimation of Glucosinolate Contents in Edible Organs of Brassica oleracea Subspecies
Previous Article in Journal
New Fluorescence Probes for Biomolecules
Article Menu

Export Article

Open AccessCommunication
Molecules 2015, 20(7), 13080-13088; doi:10.3390/molecules200713080

The Complete Chloroplast Genome of Capsicum annuum var. glabriusculum Using Illumina Sequencing

1
National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Jeonju 560-500, Korea
2
Department of Forest Genetic Resources, Korea Forest Research Institute, Suwon 441-350, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 22 May 2015 / Revised: 6 July 2015 / Accepted: 16 July 2015 / Published: 20 July 2015
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [906 KB, uploaded 20 July 2015]   |  

Abstract

Chloroplast (cp) genome sequences provide a valuable source for DNA barcoding. Molecular phylogenetic studies have concentrated on DNA sequencing of conserved gene loci. However, this approach is time consuming and more difficult to implement when gene organization differs among species. Here we report the complete re-sequencing of the cp genome of Capsicum pepper (Capsicum annuum var. glabriusculum) using the Illumina platform. The total length of the cp genome is 156,817 bp with a 37.7% overall GC content. A pair of inverted repeats (IRs) of 50,284 bp were separated by a small single copy (SSC; 18,948 bp) and a large single copy (LSC; 87,446 bp). The number of cp genes in C. annuum var. glabriusculum is the same as that in other Capsicum species. Variations in the lengths of LSC; SSC and IR regions were the main contributors to the size variation in the cp genome of this species. A total of 125 simple sequence repeat (SSR) and 48 insertions or deletions variants were found by sequence alignment of Capsicum cp genome. These findings provide a foundation for further investigation of cp genome evolution in Capsicum and other higher plants. View Full-Text
Keywords: Capsicum; bird pepper; chloroplast; DNA sequencing Capsicum; bird pepper; chloroplast; DNA sequencing
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Raveendar, S.; Na, Y.-W.; Lee, J.-R.; Shim, D.; Ma, K.-H.; Lee, S.-Y.; Chung, J.-W. The Complete Chloroplast Genome of Capsicum annuum var. glabriusculum Using Illumina Sequencing. Molecules 2015, 20, 13080-13088.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top