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Molecules 2015, 20(6), 9847-9861; doi:10.3390/molecules20069847

Synthesis of S-Layer Conjugates and Evaluation of Their Modifiability as a Tool for the Functionalization and Patterning of Technical Surfaces

1
Helmholtz-Zentrum Dresden-Rossendorf, Helmholtz-Institute Freiberg for Resource Technology, Halsbruecker Strasse 34, 09533 Freiberg, Germany
2
Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstr. 400, 01328 Dresden, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Scott Reed
Received: 26 January 2015 / Accepted: 20 May 2015 / Published: 27 May 2015
(This article belongs to the Special Issue Bioconjugations)
View Full-Text   |   Download PDF [1349 KB, uploaded 28 May 2015]   |  

Abstract

Chemical functional groups of surface layer (S-layer) proteins were chemically modified in order to evaluate the potential of S-layer proteins for the introduction of functional molecules. S-layer proteins are structure proteins that self-assemble into regular arrays on surfaces. One general feature of S-layer proteins is their high amount of carboxylic and amino groups. These groups are potential targets for linking functional molecules, thus producing reactive surfaces. In this work, these groups were conjugated with the amino acid tryptophan. In another approach, SH-groups were chemically inserted in order to extend the spectrum of modifiable groups. The amount of modifiable carboxylic groups was further evaluated by potentiometric titration in order to evaluate the potential efficiency of S-layer proteins to work as matrix for bioconjugations. The results proved that S-layer proteins can work as effective matrices for the conjugation of different molecules. The advantage of using chemical modification methods over genetic methods lies in its versatile usage enabling the attachment of biomolecules, as well as fluorescent dyes and inorganic molecules. Together with their self-assembling properties, S-layer proteins are suitable as targets for bioconjugates, thus enabling a nanostructuring and bio-functionalization of surfaces, which can be used for different applications like biosensors, filter materials, or (bio)catalytic surfaces. View Full-Text
Keywords: EDC; modified surface-layer proteins; potentiometric titration; modification rate; bio-functionalization of surfaces; chemical modification; immobilization EDC; modified surface-layer proteins; potentiometric titration; modification rate; bio-functionalization of surfaces; chemical modification; immobilization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Weinert, U.; Pollmann, K.; Barkleit, A.; Vogel, M.; Günther, T.; Raff, J. Synthesis of S-Layer Conjugates and Evaluation of Their Modifiability as a Tool for the Functionalization and Patterning of Technical Surfaces. Molecules 2015, 20, 9847-9861.

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