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Molecules 2015, 20(5), 8094-8106; doi:10.3390/molecules20058094

Effect of Chum Salmon Egg Lectin on Tight Junctions in Caco-2 Cell Monolayers

1
Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan
2
Department of Biochemistry and Microbiology, Nelson Mandel Metropolitan University, Port Elizabeth 6031, South Africa
*
Author to whom correspondence should be addressed.
Academic Editor: Els Van Damme
Received: 8 March 2015 / Revised: 20 April 2015 / Accepted: 30 April 2015 / Published: 5 May 2015
(This article belongs to the Special Issue Protein-Carbohydrate Interactions, and Beyond)
View Full-Text   |   Download PDF [1473 KB, uploaded 5 May 2015]   |  

Abstract

The effect of a chum salmon egg lectin (CSL3) on tight junction (TJ) of Caco-2 cell monolayers was investigated. The lectin opened TJ as indicated by the decrease of the transepithelial electrical resistance (TER) value and the increase of the permeation of lucifer yellow, which is transported via the TJ-mediated paracellular pathway. The effects of CSL3 were inhibited by the addition of 10 mM L-rhamnose or D-galactose which were specific sugars for CSL3. The lectin increased the intracellular Ca2+ of Caco-2 cell monolayers, that could be inhibited by the addition of L-rhamnose. The fluorescence immunostaining of β-actin in Caco-2 cell monolayers revealed that the cytoskeleton was changed by the CSL3 treatment, suggesting that CSL3 depolymerized β-actin to cause reversible TJ structural and functional disruption. Although Japanese jack bean lectin and wheat germ lectin showed similar effects in the decrease of the TER values and the increase of the intracellular Ca2+, they could not be inhibited by the same concentrations of simple sugars, such as D-glucose and N-acetyl-D-glucosamine. View Full-Text
Keywords: Caco-2 cell; intestinal transport; lectin; tight junction; transepithelial transport Caco-2 cell; intestinal transport; lectin; tight junction; transepithelial transport
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Nemoto, R.; Yamamoto, S.; Ogawa, T.; Naude, R.; Muramoto, K. Effect of Chum Salmon Egg Lectin on Tight Junctions in Caco-2 Cell Monolayers. Molecules 2015, 20, 8094-8106.

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