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Molecules 2015, 20(5), 7637-7656; doi:10.3390/molecules20057637

Lectin Engineering, a Molecular Evolutionary Approach to Expanding the Lectin Utilities

1
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China
2
Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Central-2, 1-1-1, Umezono, Tsukuba, Ibaraki 305-8568, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Els Van Damme and Kristof De Schutter
Received: 31 March 2015 / Revised: 20 April 2015 / Accepted: 20 April 2015 / Published: 27 April 2015
(This article belongs to the Special Issue Protein-Carbohydrate Interactions, and Beyond)
View Full-Text   |   Download PDF [1130 KB, uploaded 27 April 2015]   |  

Abstract

In the post genomic era, glycomics—the systematic study of all glycan structures of a given cell or organism—has emerged as an indispensable technology in various fields of biology and medicine. Lectins are regarded as “decipherers of glycans”, being useful reagents for their structural analysis, and have been widely used in glycomic studies. However, the inconsistent activity and availability associated with the plant-derived lectins that comprise most of the commercially available lectins, and the limit in the range of glycan structures covered, have necessitated the development of innovative tools via engineering of lectins on existing scaffolds. This review will summarize the current state of the art of lectin engineering and highlight recent technological advances in this field. The key issues associated with the strategy of lectin engineering including selection of template lectin, construction of a mutagenesis library, and high-throughput screening methods are discussed. View Full-Text
Keywords: lectin engineering; molecular evolution; carbohydrate specificity; scaffold; error-prone polymerase-chain reaction (PCR) lectin engineering; molecular evolution; carbohydrate specificity; scaffold; error-prone polymerase-chain reaction (PCR)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Hu, D.; Tateno, H.; Hirabayashi, J. Lectin Engineering, a Molecular Evolutionary Approach to Expanding the Lectin Utilities. Molecules 2015, 20, 7637-7656.

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