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Molecules 2015, 20(5), 7574-7585; doi:10.3390/molecules20057574

Nitric Oxide Plays a Central Role in Water Stress-Induced Tanshinone Production in Salvia miltiorrhiza Hairy Roots

1
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
2
College of Biological Science & Engineering, Shaanxi University of Technology, Hanzhong 723000, China
3
Tasly R&D Institute, Tasly Holding Group Co. Ltd, Tianjin 300410, China
4
Tianjin Tasly Modern TCM Resources Co., Ltd., Tianjin 300402, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Claudio Battilocchio
Received: 24 February 2015 / Revised: 7 April 2015 / Accepted: 9 April 2015 / Published: 24 April 2015
(This article belongs to the Special Issue Nitric Oxide (NO) Release Chemistry)
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Abstract

Nitric oxide (NO), a well-known signaling molecule plays an important role in abiotic and biotic stress-induced production of plant secondary metabolites. In this study, roles of NO in water stress-induced tanshinone production in Salvia miltiorrhiza hairy roots were investigated. The results showed that accumulations of four tanshinone compounds in S. miltiorrhiza hairy roots were significantly stimulated by sodium nitroprusside (SNP, a NO donor) at 100 μM. Effects of SNP were just partially arrested by the mevalonate (MVA) pathway inhibitor (mevinolin), but were completely inhibited by the 2-C-methyl-d-erythritol-4-phosphate pathway (MEP) inhibitor (fosmidomycin). The increase of tanshinone accumulation and the up-regulation of HMGR and DXR expression by PEG and ABA treatments were partially inhibited by an inhibitor of NO biosynthesis (Nω-nitro-L-arginine methyl ester (L-NAME)) and a NO scavenger (2-(4-Carboxyphenyl)- 4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO)). Simultaneously, NO generation in the hairy roots was triggered by PEG and ABA, and the effects were also arrested by c-PTIO and L-NAME. These results indicated that NO signaling probably plays a central role in water stress-induced tanshinone production in S. miltiorrhiza hairy roots. SNP mainly stimulated the MEP pathway to increase tanshinone accumulation. View Full-Text
Keywords: nitric oxide; tanshinone; Salvia miltiorrhiza; polyethylene glycol; abscisic acid nitric oxide; tanshinone; Salvia miltiorrhiza; polyethylene glycol; abscisic acid
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Du, X.; Zhang, C.; Guo, W.; Jin, W.; Liang, Z.; Yan, X.; Guo, Z.; Liu, Y.; Yang, D. Nitric Oxide Plays a Central Role in Water Stress-Induced Tanshinone Production in Salvia miltiorrhiza Hairy Roots. Molecules 2015, 20, 7574-7585.

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