Biological Activities and Cytotoxicity of Diterpenes from Copaifera spp. Oleoresins
Abstract
:1. Introduction
2. Results and Discussion
2.1. Inhibition of Tyrosinase
2.2. Lipoxygenase Inhibition
2.3. Hemolytic Activity
2.4. Cytotoxicity
Kolavic Acid 15 Methyl Ester | Copalic Acid | Hardwickiic Acid | Kaurenoic Acid | 3-Hydroxy-Copalic Acid | 3-Acetoxy-Copalic Acid | |
---|---|---|---|---|---|---|
AGP01 | 17.4 ± 6.7 | NT | NT | 28.1 ± 4.3 | 8.5 ± 13.1 | 13.1 ± 5.3 |
HCT-116 | ≤0.01 | ≤0.01 | ≤0.01 | 0.27 ± 4.3 | 4.7 ± 5.6 | ≤0.01 |
NIHOVCAR | ≤0.01 | ≤0.01 | ≤0.01 | ≤0.01 | ≤0.01 | 2.0 ± 3.5 |
SF295 | ≤0.01 | ≤0.01 | ≤0.01 | 28.0 ± 6.5 | 20.5 ± 5.0 | 17.7 ± 0.9 |
MCF7 | ≤0.01 | ≤0.01 | ≤0.01 | ≤0.01 | ≤0.01 | ≤0.01 |
SKMELL28 | ≤0.01 | ≤0.01 | ≤0.01 | ≤0.01 | ≤0.01 | ≤0.01 |
2.5. Inhibition of Nitric Oxide Production
2.6. Assay for Cytokine Production
3. Materials and Methods
3.1. Experimental Procedures
3.2. Plant Material
3.3. Extraction, Isolation and Structural Elucidation
3.4. Isolated Compounds Data
3.5. Cell Culture
3.6. Hemolysis Test
3.7. NO• Production Assay
3.8. Measurement of Cytokines
3.9. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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De S. Vargas, F.; D. O. de Almeida, P.; Aranha, E.S.P.; De A. Boleti, A.P.; Newton, P.; De Vasconcellos, M.C.; Junior, V.F.V.; Lima, E.S. Biological Activities and Cytotoxicity of Diterpenes from Copaifera spp. Oleoresins. Molecules 2015, 20, 6194-6210. https://doi.org/10.3390/molecules20046194
De S. Vargas F, D. O. de Almeida P, Aranha ESP, De A. Boleti AP, Newton P, De Vasconcellos MC, Junior VFV, Lima ES. Biological Activities and Cytotoxicity of Diterpenes from Copaifera spp. Oleoresins. Molecules. 2015; 20(4):6194-6210. https://doi.org/10.3390/molecules20046194
Chicago/Turabian StyleDe S. Vargas, Fabiano, Patrícia D. O. de Almeida, Elenn Suzany P. Aranha, Ana Paula De A. Boleti, Peter Newton, Marne C. De Vasconcellos, Valdir F. Veiga Junior, and Emerson S. Lima. 2015. "Biological Activities and Cytotoxicity of Diterpenes from Copaifera spp. Oleoresins" Molecules 20, no. 4: 6194-6210. https://doi.org/10.3390/molecules20046194