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Molecules 2015, 20(4), 5714-5728; doi:10.3390/molecules20045714

Strophalloside Induces Apoptosis of SGC-7901 Cells through the Mitochondrion-Dependent Caspase-3 Pathway

1
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571199, China
2
Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou 571199, China
3
College of Pharmacy and Chemistry, DaLi University, Dali 671099, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 11 February 2015 / Revised: 18 March 2015 / Accepted: 25 March 2015 / Published: 31 March 2015
(This article belongs to the Section Medicinal Chemistry)
View Full-Text   |   Download PDF [4955 KB, uploaded 31 March 2015]   |  

Abstract

Cardenolides with special chemical structures have been considered as effective anti-cancer drugs in clinic trials. Strophalloside is a cardenolide we recently isolated from Antiaris toxicaria obtained from Hainan, China. The aim of this study was to investigate the possible anticancer effects induced by strophalloside and the underlying molecular mechanism. Gastric carcinoma SGC-7901 cells were treated with strophalloside at various concentrations for different times, and resulting cell viability was determined by the MTT assay, and the motility and invasion of tumor cells were assessed by the Transwell chamber assay. Apoptosis were measured by Annexin V-FITC/PI and Hoechst staining. The changes of mitochondrial transmembrane potential were examined by a JC-1 kit. The expressions of pro-apoptotic protein cytochrome c, caspase-3 and caspase-9 were detected by western blotting analysis. The results showed that strophalloside was capable of reducing cell viability, inhibiting cell growth, and suppressing cell migration and invasion in a time- and dose-dependent manner. Mitochondrial membrane potential declined and the concentration of cytochrome c increased in cytoplasm and caspase-3 and caspase-9 were cleaved into activated states, suggesting that cytochrome c was released from the mitochondrion to cytoplasm and finally activated the caspase-dependent apoptosis pathway. Our results indicate that strophalloside is a potential anticancer drug. View Full-Text
Keywords: strophalloside; gastric carcinoma; mitochondrion-mediated pathway; apoptosis; cytochrome c; caspase strophalloside; gastric carcinoma; mitochondrion-mediated pathway; apoptosis; cytochrome c; caspase
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zhang, X.-J.; Mei, W.-L.; Tan, G.-H.; Wang, C.-C.; Zhou, S.-L.; Huang, F.-R.; Chen, B.; Dai, H.-F.; Huang, F.-Y. Strophalloside Induces Apoptosis of SGC-7901 Cells through the Mitochondrion-Dependent Caspase-3 Pathway. Molecules 2015, 20, 5714-5728.

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