Investigation of Structural Mimetics of Natural Phosphate Ion Binding Motifs
AbstractPhosphates are ubiquitous in biology and nearly half of all proteins interact with their partners by means of recognition of phosphate residues. Therefore, a better understanding of the phosphate ion binding by peptidic structures is highly desirable. Two new receptors have been designed and synthesized and their anion binding properties in an acetonitrile solution have been determined. The structure of hosts mimics a part of the kinase active site that is responsible for the recognition of the phosphate residue. New hosts contain additional free amino groups with the aim to facilitate coordination of protonated anions, such as dihydrogen phosphate. According to spectrophotometric measurements, stepwise 1:1 and 1:2 binding modes have been observed for both receptors in the presence of acetate, hydrogen sulfate and dihydrogen phosphate. Compared with the acyclic receptor, the macrocyclic receptor has demonstrated a remarkably enhanced selectivity for dihydrogen phosphate over other anions. Fluorometric measurements have revealed different responses of the acyclic and macrocyclic receptors towards anions. However, in both cases, a 5–8 nm hypsochromic shift of fluorescence maximum has been observed upon interaction of acetate and dihydrogen phosphate with receptors. View Full-Text
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Kataev, E.A.; Shumilova, T.A. Investigation of Structural Mimetics of Natural Phosphate Ion Binding Motifs. Molecules 2015, 20, 3354-3370.
Kataev EA, Shumilova TA. Investigation of Structural Mimetics of Natural Phosphate Ion Binding Motifs. Molecules. 2015; 20(2):3354-3370.Chicago/Turabian Style
Kataev, Evgeny A.; Shumilova, Tatiana A. 2015. "Investigation of Structural Mimetics of Natural Phosphate Ion Binding Motifs." Molecules 20, no. 2: 3354-3370.