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Molecules 2015, 20(2), 3206-3220; doi:10.3390/molecules20023206

Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid Contents

1
Drug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia
2
Faculty of Applied Sciences, Universiti Teknologi Mara, Shah Alam 40450, Malaysia
3
Laboratory of Natural Products Chemistry, Faculty of Pharmacy, Halu Oleo University, Kendari 93232, Indonesia
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 13 November 2014 / Accepted: 30 January 2015 / Published: 16 February 2015
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [1667 KB, uploaded 16 February 2015]   |  

Abstract

The crude methanol extracts and fractions of the root and stem barks of Dillenia serrata Thunb. showed 64% to 73% inhibition on the production of prostaglandin E2 (PGE2) in lipopolysaccharide-induced human whole blood using a radioimmunoassay technique. Three triterpenoids isolated from the root bark of the plant, koetjapic (1), 3-oxoolean-12-en-30-oic (2), and betulinic (3) acids, exhibited significant concentration-dependent inhibitory effects on PGE2 production with IC50 values of 1.05, 1.54, and 2.59 μM, respectively, as compared with the positive control, indomethacin (IC50 = 0.45 μM). Quantification of compounds 1 and 3 in the methanol extracts and fractions were carried out by using a validated reversed-phase high performance liquid chromatography (RP-HPLC) method. The ethyl acetate fraction of the stem bark showed the highest content of both compound 1 (15.1%) and compound 3 (52.8%). The strong inhibition of the extracts and fractions on cyclooxygenase-2 (COX-2) enzymatic activity was due to the presence of their major constituents, especially koetjapic and betulinic acids. View Full-Text
Keywords: Dilleniaceae; Dillenia serrata; triterpenoids; prostaglandin E2; HPLC Dilleniaceae; Dillenia serrata; triterpenoids; prostaglandin E2; HPLC
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Jalil, J.; Sabandar, C.W.; Ahmat, N.; Jamal, J.A.; Jantan, I.; Aladdin, N.-A.; Muhammad, K.; Buang, F.; Mohamad, H.F.; Sahidin, I. Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid Contents. Molecules 2015, 20, 3206-3220.

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