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Expression of Concern published on 2 June 2016, see Molecules 2016, 21(6), 725.

Editorial published on 10 January 2018, see Molecules 2018, 23(1), 136.

Open AccessArticle
Molecules 2015, 20(12), 22170-22187; doi:10.3390/molecules201219819

Helleborus purpurascens—Amino Acid and Peptide Analysis Linked to the Chemical and Antiproliferative Properties of the Extracted Compounds

National Institute for Research and Development in Electrochemistry and Condensed Matter—INCEMC, Timisoara 300224, Romania
University Politehnica Timisoara, 2 Piata Victoriei, Timisoara 300006, Romania
University “Eftimie Murgu”, Resita, 1-4 Traian Vuia, Resita 320085, Romania
Department of Morphological Sciences, University of Medicine and Pharmacy “Carol Davila”, 8 Eroilor Sanitari Blvd, Sector 5, Bucharest 050474, Romania
“Victor Babes” National Institute of Pathology, 99-101, Splaiul Independentei, Sector 5, Bucharest 050096, Romania
Author to whom correspondence should be addressed.
Academic Editor: Isabel C. F. R. Ferreira
Received: 1 October 2015 / Revised: 27 November 2015 / Accepted: 27 November 2015 / Published: 11 December 2015
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [5107 KB, uploaded 11 December 2015]   |  


There is a strong drive worldwide to discover and exploit the therapeutic potential of a large variety of plants. In this work, an alcoholic extract of Helleborus purpurascens (family Ranunculaceae) was investigated for the identification of amino acids and peptides with putative antiproliferative effects. In our work, a separation strategy was developed using solvents of different polarity in order to obtain active compounds. Biochemical components were characterized through spectroscopic (mass spectroscopy) and chromatographic techniques (RP-HPLC and GC-MS). The biological activity of the obtained fractions was investigated in terms of their antiproliferative effects on HeLa cells. Through this study, we report an efficient separation of bioactive compounds (amino acids and peptides) from a plant extract dependent on solvent polarity, affording fractions with unaffected antiproliferative activities. Moreover, the two biologically tested fractions exerted a major antiproliferative effect, thereby suggesting potential anticancer therapeutic activity. View Full-Text
Keywords: antiproliferative activiy; chromatographic techniques; hellebore; thionins; mass spectroscopy antiproliferative activiy; chromatographic techniques; hellebore; thionins; mass spectroscopy

Figure 1a

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Segneanu, A.-E.; Grozescu, I.; Cziple, F.; Berki, D.; Damian, D.; Niculite, C.M.; Florea, A.; Leabu, M. Helleborus purpurascens—Amino Acid and Peptide Analysis Linked to the Chemical and Antiproliferative Properties of the Extracted Compounds. Molecules 2015, 20, 22170-22187.

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