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Molecules 2015, 20(11), 20409-20425; doi:10.3390/molecules201119702

Sensitive and Rapid UHPLC-MS/MS for the Analysis of Tomato Phenolics in Human Biological Samples

1
Department of Nutrition and Food Science-XARTA-INSA, School of Pharmacy, University of Barcelona, Barcelona 08028, Spain
2
Centre for Biomedical Network Research on the Pathophysiology of Obesity and Nutrition (CIBEROBN). Carlos III Health Institute, Madrid 28029, Spain
3
Biomedical Research Institute (IBIMA), Service of Endocrinology and Nutrition, Hospital Virgen de la Victoria, Teatinos Campus, University of Malaga, Malaga 29010, Spain
4
Scientific and Technological Centers of the University of Barcelona (CCiTUB), Barcelona 08028, Spain
5
Department of Internal Medicine, Hospital Clinic, Institute of Biomedical Investigation August Pi i Sunyer (IDIBAPS), University of Barcelona, Barcelona 08036, Spain
6
INRA, UMR1083 Sciences for Oenology, 2 place Pierre Viala, Montpellier Cedex 34060, France
*
Author to whom correspondence should be addressed.
Academic Editors: Luis Cisneros-Zevallos and Daniel Jacobo-Velazquez
Received: 18 August 2015 / Revised: 19 October 2015 / Accepted: 4 November 2015 / Published: 16 November 2015
(This article belongs to the Special Issue Recent Advances in Plant Phenolics)
View Full-Text   |   Download PDF [799 KB, uploaded 16 November 2015]   |  

Abstract

An UHPLC-MS/MS method for the quantification of tomato phenolic metabolites in human fluids was optimized and validated, and then applied in a pilot dietary intervention study with healthy volunteers. A 5-fold gain in speed (3.5 min of total run); 7-fold increase in MS sensitivity and 2-fold greater efficiency (50% peak width reduction) were observed when comparing the proposed method with the reference-quality HPLC-MS/MS system, whose assay performance has been previously documented. The UHPLC-MS/MS method led to an overall improvement in the limits of detection (LOD) and quantification (LOQ) for all the phenolic compounds studied. The recoveries ranged between 68% and 100% in urine and 61% and 100% in plasma. The accuracy; intra- and interday precision; and stability met with the acceptance criteria of the AOAC International norms. Due to the improvements in the analytical method; the total phenolic metabolites detected in plasma and urine in the pilot intervention study were 3 times higher than those detected by HPLC-MS/MS. Comparing with traditional methods; which require longer time of analysis; the methodology described is suitable for the analysis of phenolic compounds in a large number of plasma and urine samples in a reduced time frame. View Full-Text
Keywords: polyphenol metabolites; validation; tomato; sauce; microbiota; metabolites; biosamples; blood; urine polyphenol metabolites; validation; tomato; sauce; microbiota; metabolites; biosamples; blood; urine
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Martínez-Huélamo, M.; Tulipani, S.; Jáuregui, O.; Valderas-Martinez, P.; Vallverdú-Queralt, A.; Estruch, R.; Torrado, X.; Lamuela-Raventós, R.M. Sensitive and Rapid UHPLC-MS/MS for the Analysis of Tomato Phenolics in Human Biological Samples. Molecules 2015, 20, 20409-20425.

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