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Molecules 2015, 20(11), 20219-20229; doi:10.3390/molecules201119687

Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers

1
Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China
2
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau (SAR) 999078, China
3
Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran 14359-44711, Iran
4
Department of Biochemistry, School of Life Sciences & the State Key Laboratory of Medical Genetics, Central South University, Changsha 410013, China
5
Judicial Authentication Center, Sichuan Medical University, Luzhou 646000, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 21 August 2015 / Revised: 18 October 2015 / Accepted: 3 November 2015 / Published: 10 November 2015
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [5344 KB, uploaded 10 November 2015]   |  

Abstract

The evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random amplified polymorphic DNA (RAPD) derived fragments into T-vector, and developed sequence-characterized amplified region (SCAR) markers. These markers have been deposited in GenBank database with the accession numbers KP641310, KP641311, KP641312 and KP641313 respectively. The BLAST search of database confirmed the novelty of these markers. The four SCAR markers, namely ZZH11, ZZH31, ZZH41 and ZZH51 can specifically recognize the genetic materials of G. jasminoides from other plant species. Moreover, SCAR marker ZZH31 can be used to distinguish G. jasminoides Ellis var. grandiflora Nakai from other G. jasminoides on the market. Together, this study has developed four stably molecular SCAR markers by improved RAPD-derived DNA markers for the genetic identification and authentication, and for ecological conservation of medicinal and ornamental plant G. jasminoides. View Full-Text
Keywords: G. jasminoides Ellis var. grandiflora Nakai; Gardenia jasminoides; random amplified polymorphic DNA; sequence-characterized amplified region; cloning; genetic authentication G. jasminoides Ellis var. grandiflora Nakai; Gardenia jasminoides; random amplified polymorphic DNA; sequence-characterized amplified region; cloning; genetic authentication
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Mei, Z.; Zhou, B.; Wei, C.; Cheng, J.; Imani, S.; Chen, H.; Fu, J. Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers. Molecules 2015, 20, 20219-20229.

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