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Molecules 2015, 20(11), 19929-19935; doi:10.3390/molecules201119669

Development and Characterization of Polymorphic Microsatellite Markers for Sedum sarmentosum (Crassulaceae) and Their Cross-Species Transferability

College of Pharmacy, South Central University for Nationalities, 708 Minyuan Road, Wuhan 430074, China
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Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 17 August 2015 / Revised: 8 October 2015 / Accepted: 10 October 2015 / Published: 5 November 2015
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [191 KB, uploaded 5 November 2015]

Abstract

Sedum sarmentosum is an important Chinese medicinal herb that exhibits anti-inflammatory, anti-angiogenic and anti-nociceptive properties. However, little is known about its genetic background. The first set of 14 microsatellite markers were isolated and characterized for S. sarmentosum using an SSR-enriched library. Fourteen polymorphic microsatellite markers were acquired with satisfactory amplifications and a polymorphic pattern in 48 S. sarmentosum individuals. The number of alleles ranged from 3 to 15. The observed and expected heterozygosities varied from 0.0833 to 0.8750 and 0.2168 to 0.9063, respectively. Two loci showed significant departure from the Hardy-Weinberg equilibrium. Cross-species amplification was carried out in other Sedum species. High rates of cross-species amplification were observed. The transferability value ranged from 85.7% in S. lineare to 64.3% in S. ellacombianum. These markers will be valuable for studying the genetic variation, population structure and germplasm characterization of S. sarmentosum and related Sedum species. View Full-Text
Keywords: Sedum sarmentosum; microsatellite markers; polymorphism; genetic diversity; transferability Sedum sarmentosum; microsatellite markers; polymorphism; genetic diversity; transferability
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Xu, J.; Hou, F.-Y.; Wan, D.-R.; Wang, S.; Xu, D.-M.; Yang, G.-Z. Development and Characterization of Polymorphic Microsatellite Markers for Sedum sarmentosum (Crassulaceae) and Their Cross-Species Transferability. Molecules 2015, 20, 19929-19935.

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