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Erratum published on 21 July 2016, see Molecules 2016, 21(7), 957.

Open AccessCommunication
Molecules 2015, 20(11), 19907-19913; doi:10.3390/molecules201119672

Radical Scavenging by Acetone: A New Perspective to Understand Laccase/ABTS Inactivation and to Recover Redox Mediator

1,†,* , 2,†
,
1
,
1
and
3,*
1
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China
2
School of Chemistry and Environmental Engineering, Hunan City University, Yiyang 413000, China
3
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 30 July 2015 / Revised: 27 October 2015 / Accepted: 28 October 2015 / Published: 4 November 2015
(This article belongs to the Section Bioorganic Chemistry)
View Full-Text   |   Download PDF [1000 KB, uploaded 21 July 2016]   |  

Abstract

The biosynthetic utilization of laccase/mediator system is problematic because the use of organic cosolvent causes significant inhibition of laccase activity. This work explored how the organic cosolvent impacts on the laccase catalytic capacity towards 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) in aqueous solution. Effects of acetone on the kinetic constants of laccase were determined and the results showed Km and Vmax varied exponentially with increasing acetone content. Acetone as well as some other cosolvents could transform ABTS radicals into its reductive form. The content of acetone in media significantly affected the radical scavenging rates. Up to 95% of the oxidized ABTS was successfully recovered in 80% (v/v) acetone in 60 min. This allows ABTS recycles at least six times with 70%–75% of active radicals recovered after each cycle. This solvent-based recovery strategy may help improve the economic feasibility of laccase/ABTS system in biosynthesis. View Full-Text
Keywords: laccase; ABTS; inhibition; radical scavenging; recovery laccase; ABTS; inhibition; radical scavenging; recovery
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Liu, H.; Zhou, P.; Wu, X.; Sun, J.; Chen, S. Radical Scavenging by Acetone: A New Perspective to Understand Laccase/ABTS Inactivation and to Recover Redox Mediator. Molecules 2015, 20, 19907-19913.

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