Qualitative and Quantitative Analysis of the Major Constituents in Shexiang Tongxin Dropping Pill by HPLC-Q-TOF-MS/MS and UPLC-QqQ-MS/MS
Abstract
:1. Introduction
2. Results and Discussion
2.1. Optimization of HPLC Conditions
2.2. HPLC-Q-TOF-MS/MS Qualitative Analysis of Chemical Constituents in STP
No. | Source | Identification | Rt (min) | Formula | Negative Ion (m/z) | |||
---|---|---|---|---|---|---|---|---|
[M − H]− | Quasi-Molecular Ion | Error (ppm) | MSMS (m/z) | |||||
1 | C & B | Taurine | 3.7 | C2H7NO3S | 124.0063 | 124.0065 | 2 | |
2 | R | Salvianolic acid A | 23.8 | C26H22O10 | 493.1129 | 493.1133 | 1 | |
3 | C & B | Bilirubin | 24.2 | C33H35N4O6 | 583.2551 | 583.2518 | 6 | 583.2518, 469.3944,356.4968, 243.8168 |
4 | G | Ginsenoside Rk3 | NA | C36H60O8 | 619.4204 | NA | ||
5 | G | Ginsenoside Rd | 24.9 | C48H82O18 | 945.5417 | 945.5405 | 1 | |
6 | R | Tanshinaldehyde | NA | C19H16O4 | 307.0965 | NA | ||
7 | G | Ginsenoside Rg6 | NA | C42H70O12 | 765.4784 | NA | ||
8 a | R | Salvianolic acid B | 25.5 | C36H30O16 | 717.1450 | 717.1454 | 0 | 717.1454, 537.0995, 527.9935, 493.1069, 339.0521, 321.0405, 295.0633 |
9 a | V | Arenobufagin | 28.7 | C24H32O6 | 415.2115 | 415.2115 | 0 | 415.2115, 397.2063, 371.2165, 353.2112, 277.1492, 196.5435, 151.0702 |
10 a | C & B | TDCANa | 33.8 | C26H44NNaO6S | 498.2884 | 498.2885 | 0 | 498.2907, 475.3216, 458.8943, 391.5463 |
11 a | C & B | TCA | 33.8 | C26H44NO7S | 514.2833 | 514.2856 | 4 | 514.2856, 496.2486, 482.3441, 124.1263 |
12 | G | Ginsenoside Rf | NA | C35H76O19 | 799.4897 | NA | ||
13 | V | Gamabufotalin | NA | C24H34O5 | 401.2323 | NA | ||
14 | G | Notoginsenoside R2 | NA | C41H70O13 | 769.4733 | NA | ||
15 | G | Ginsenoside Rb1 | 40.5 | C54H92O23 | 1107.5946 | 1107.5927 | 2 | 1107.5927, 945.5389, 783.4901, 765.4580, 621.4343, 553.2887, 472.2575, 459.3803, 323.0972, 263.0742, 221.0678, 179.0538, 179.0538, 161.0395, 143.0272, 131.0323, 125.0221, 119.0351, 113.0260, 101.0208 |
16 | V | Bufotalin | 41.1 | C26H36O6 | 443.2428 | 443.2452 | 5 | 443.2452, 407.2732, 380.9754, 375.2916, 256.8133, 203.8251, 167.9242, 138.0325 |
17 a | G | Ginsenoside Rh1 | 41.8 | C37H64O11 | 683.4403 | 683.4384 | 3 | 683.4384, 637.4330, 475.3704, 179.0470, 161.0398 |
18 | C & B | GCA | 42.6 | C26H43NO6 | 464.3007 | 464.3015 | 2 | |
19 a | C & B | TDCA | 42.8 | C26H45NO6S | 498.2884 | 498.2907 | 5 | 498.2907, 464.2945, 451.3111, 391.1261, 321.1686, 201.2573 |
20 | V | Resibufagin | NA | C24H30O5 | 397.2010 | NA | ||
21 | G | Ginsenoside Rb2 | 43.7 | C53H90O22 | 1077.5840 | 1077.5795 | 4 | 1077.5795, 945.5318, 915.1144, 783.0026, 765.1212, 621.3652, 311.9821, 293.6514, 191.8123, 149.1853 |
22 | G | Ginsenoside Re | 47.2 | C48H82O18 | 945.5417 | 945.5433 | 2 | 945.5433, 880.9436, 799.2672, 765.1661, 637.2542, 475.2767, 218.9817 |
23 | G | Ginsenoside Rk1 | NA | C42H70O12 | 765.4784 | NA | ||
24 a | V | Bufalin | NA | C24H34O4 | 385.2373 | NA | ||
25 a | C & B | CA | 52.1 | C24H40O5 | 407.2792 | 407.2811 | 5 | 407.2811, 389.2677, 363.2526, 325.6244, 289.2744, 233.8977, 215.3690, 205.9169 |
26 | V | Argentinogenin-3-lutarate-arginine | 53.6 | C38H58N408 | 697.4171 | 697.4121 | 7 | 697.4121, 651.4153, 535.5406, 489.3649, 179.0546 |
27 | C & B | TLCA | 55.0 | C22H44NO10 | 482.2960 | 482.2918 | 9 | |
28 | G | Ginsenoside Rg1 | 55.3 | C43H72O14 | 811.4838 | 811.4813 | 3 | 811.4813, 775.8988, 765.4577, 619.3911, 421.2024, 391.2774 |
29 | C & B | DCA | 55.8 | C24H40O4 | 391.2843 | 391.2854 | 3 | |
30 | C & B | GCDGA | 56.2 | C26H43NO5 | 448.3058 | 448.3065 | 2 | 448.3065, 409.2741, 391.2853, 389.2658, 365.3004 |
31 a | C & B | UDCA | 56.8 | C24H40O4 | 391.2843 | 391.2848 | 1 | 409.2741, 391.2739, 373.8139, 354.4757, 152.9965 |
32 a | C & B | HDCA | 57.4 | C24H40O4 | 391.2843 | 391.2851 | 2 | 409.2741, 391.2853, 389.2658, 118.1248 |
33 a | V | Resibufogenin | NA | C24H32O4 | 383.2217 | NA | ||
34 | G | Ginsenoside Rs3 | NA | C44H74O14 | 825.4995 | NA | ||
35 a | V | Cinobufagin | NA | C26H34O6 | 441.2272 | NA | ||
36 | G | Chikusetsusaponin Iva | 61.6 | C42H66O14 | 793.4374 | 793.4330 | 6 | 793.4330, 598.6542, 481.7612, 524.4589 |
37 | G | Ginsenoside 20S-Rg3 | NA | C42H72O13 | 783.4889 | NA | ||
38 a | G | ginsenoside Rg2 | 68.9 | C42H72O13 | 783.4889 | 783.4873 | 2 | 783.4873, 765.1686, 617.0004, 409.2952, 391.2852, 313.1097 |
39 a | C & B | CDCA | 68.9 | C24H40O4 | 391.2843 | 391.2853 | 3 | 391.2739, 345.4757, 329.1213, 97.0589 |
40 | C & B | LCA | 89.4 | C24H40O3 | 375.2894 | 375.2888 | 2 | |
41 a | R | Tanshinone IIA | NA | C19H18O3 | 293.1172 | NA | ||
No. | Source | Identification | Rt (min) | Formula | Positive Ion (m/z) | |||
[M + H]+ | Quasi-Molecular Ion | Error (ppm) | MSMS (m/z) | |||||
1 | C & B | Taurine | 3.7 | C2H7NO3S | 126.0219 | 126.0222 | 2 | 126.0222, 115.3256, 108.8434, 97.2221, 91.7216 |
2 | R | Salvianolic acid A | NA | C26H22O10 | 495.1286 | NA | ||
3 | C & B | Bilirubin | NA | C33H35N4O6 | 584.2629 | NA | ||
4 | G | Ginsenoside Rk3 | 24.5 | C36H60O8 | 621.4361 | 621.4362 | 0 | |
5 | G | Ginsenoside Rd | 24.6 | C48H82O18 | 947.5574 | 947.5501 | 8 | |
6 | R | Tanshinaldehyde | 24.6 | C19H16O4 | 309.1121 | 309.1147 | 8 | |
7 | G | Ginsenoside Rg6 | 24.6 | C42H70O12 | 767.4940 | 767.4931 | 1 | |
8 a | R | Salvianolic acid B | 25.8 | C36H30O16 | 719.1607 | 719.1613 | 1 | 719.1613, 323.0496, 295.0572, 181.0460 |
9 a | V | Arenobufagin | 28.4 | C24H32O6 | 417.2272 | 417.2273 | 0 | 417.2273, 399.2188, 362.7495, 223.1056 |
10 a | C & B | TDCANa | 33.2 | C26H44NNaO6S | 500.4245 | NA | ||
11 a | C & B | TCA | 33.5 | C26H44NO7S | 516.2990 | 516.2984 | 1 | |
12 | G | Ginsenoside Rf | 34.0 | C35H76O19 | 801.5054 | 801.5075 | 3 | |
13 | V | Gamabufotalin | 38.9 | C24H34O5 | 403.2251 | 403.2267 | 4 | 403.2267, 385.9531, 367.1296, 349.4632, 331.8123 |
14 | G | Notoginsenoside R2 | 39.0 | C41H70O13 | 771.4889 | 771.4861 | 4 | |
15 | G | Ginsenoside Rb1 | 39.9 | C54H92O23 | 1109.6102 | 1109.6030 | 6 | 1109.6030, 935.5613, 878.7854, 646.6751, 443.3798, 407.3652, 325.1114, 217.1888, 163.0566, 145.0507, 127.0384, 85.0294 |
16 | V | Bufotalin | 41.1 | C26H36O6 | 445.2585 | 445.2585 | 0 | |
17 a | G | Ginsenoside Rh1 | NA | C37H64O11 | 685.4521 | NA | ||
18 | C & B | GCA | 42.3 | C26H43NO6 | 466.3163 | 466.3132 | 7 | 466.3132, 337.2523, 319.2353, 295.2026, 288.1602, 213.1621, 209.1339, 201.1658 |
19 a | C & B | TDCA | 42.8 | C26H45NO6S | 500.3040 | 500.3042 | 0 | |
20 | V | Resibufagin | 42.8 | C24H30O5 | 399.2166 | 399.2158 | 2 | 399.2158, 387.2519, 297.7655, 223.1439, 211.1011, 145.0999, 131.0982, 179.0841, 105.0709, 91.0525 |
21 | G | Ginsenoside Rb2 | 43.1 | C53H90O22 | 1079.5997 | 1079.5942 | 5 | |
22 | G | Ginsenoside Re | 46.5 | C48H82O18 | 947.5574 | 947.5510 | 7 | |
23 | G | Ginsenoside Rk1 | 46.6 | C42H70O12 | 767.4940 | 767.4931 | 1 | |
24 a | V | Bufalin | 49.1 | C24H34O4 | 387.253 | 387.2527 | 1 | 387.2527, 370.9842, 352.9666, 340.2608, 255.2028 |
25 a | C & B | CA | NA | C24H40O5 | 409.2949 | NA | ||
26 | V | Argentinogenin-3-lutarate-arginine | 53.2 | C38H58N408 | 699.4327 | 699.4299 | 4 | 699.4299, 681.4053, 598.7017, 582.3791, 331.2027, 278.1446, 250.1590, 175.1200, 157.1072 |
27 | C & B | TLCA | NA | C22H44NO10 | 483.3038 | NA | ||
28 | G | Ginsenoside Rg1 | NA | C43H72O14 | 813.4995 | NA | ||
29 | C & B | DCA | NA | C24H40O4 | 393.3000 | NA | ||
30 | C & B | GCDGA | 56.1 | C26H43NO5 | 450.3214 | 450.3204 | 2 | 450.3204, 415.3174, 321.2607, 278.7283, 215.1679, 175.1452, 161.1349, 147.1140, 107.0885 |
31 a | C & B | UDCA | NA | C24H40O4 | 393.3000 | NA | ||
32 a | C & B | HDCA | NA | C24H40O4 | 393.3000 | NA | ||
33 a | V | Resibufogenin | 57.7 | C24H32O4 | 385.2373 | 385.2369 | 1 | 385.2369, 367.2214, 350.2243, 341.2244, 332.2025 |
34 | G | Ginsenoside Rs3 | 57.8 | C44H74O14 | 827.5151 | 827.5165 | 2 | |
35 a | V | Cinobufagin | 57.9 | C26H34O6 | 443.2428 | 443.2425 | 1 | 443.2425, 401.2288, 385.2322, 367.2210, 349.2129, 187.1473, 151.0382 |
36 | G | Chikusetsusaponin Iva | NA | C42H66O14 | 795.4525 | NA | ||
37 | G | Ginsenoside 20S-Rg3 | 68.6 | C42H72O13 | 785.5046 | 785.5063 | 2 | 785.5063, 621.6871, 357.2785, 339.2647, 321.2458, 275.2027, 221.1526, 161.1345 |
38 a | G | ginsenoside Rg2 | NA | C42H72O13 | 785.5046 | NA | ||
39 a | C & B | CDCA | NA | C24H40O4 | 393.3000 | NA | ||
40 | C & B | LCA | NA | C24H40O3 | 377.3050 | NA | ||
41 a | R | Tanshinone IIA | 92.3 | C19H18O3 | 295.1269 | 295.1266 | 0 | 295.1266, 280.4501, 277.1173, 266.0878, 262.0933, 249.1212, 235.0725, 225.1178, 221.1251, 207.0771 |
2.2.1. Compounds from Ginseng
2.2.2. Compounds from Salvia miltiorrhiza
2.2.3. Compounds from Toad Skin Secretion (Venenum bufonis)
2.2.4. Compounds from Calculus bovis and Bear Gall
2.3. UPLC-QqQ-MS/MS Quantitative Analysis of Chemical Constituents from STP
2.4. Method Validation
2.4.1. Linearity and Detection Limit
Compound | Linear | Linear Range (μg/mL) | r | LOQ (μg/mL) | LOD (μg/mL) |
---|---|---|---|---|---|
Taurine | y = 0.0707x − 0.0005 | 10.03–0.40 | 0.9999 | 0.1300 | 0.0390 |
Ginsenoside Rg1 | y = 0.0574x − 0.0102 | 7.51–0.75 | 0.9998 | 0.6000 | 0.1800 |
Arenobufagin | y = 0.3438x − 0.0003 | 5.02–0.40 | 0.9996 | 0.0300 | 0.0090 |
TDCA | y = 0.0125x − 0.0061 | 25.28–3.75. | 0.9992 | 1.7000 | 0.5100 |
TCA | y = 0.0525x − 0.0094 | 10.53–0.75 | 0.9996 | 0.6000 | 0.1800 |
Bufalin | y = 1.1663x + 0.0624 | 4.01–0.40 | 0.9999 | 0.0032 | 0.0010 |
CA | y = 0.0909x − 0.0211 | 10.07–1.01 | 0.9998 | 0.7800 | 0.2340 |
Cinobufagin | y = 0.1767x + 0.0523 | 5.02–0.75 | 0.9998 | 0.0003 | 0.0010 |
Resibufogenin | y = 0.9504x − 0.0596 | 4.01–0.50 | 0.9993 | 0.2100 | 0.0630 |
Ginsenoside Rg3 | y = 0.048x + 0.0256 | 2.01–0.01 | 0.9994 | 0.0041 | 0.0012 |
CDCA | y = 0.2824x − 0.0241 | 5.01–0.75 | 0.9993 | 0.2900 | 0.0870 |
DCA | y = 0.2049x + 0.0145 | 4.01–0.40 | 0.9990 | 0.0050 | 0.0015 |
Tanshinone IIA | y = 34.5600x − 0.0030 | 0.05–0.01 | 0.9995 | 0.0042 | 0.0013 |
2.4.2. Precision, Repeatability, Stability and Recovery
Analyte | Repeatability | Precision RSD% | Stability | ||
---|---|---|---|---|---|
Content (mg/g) ± SD | RSD% n = 6 | Intra-Day n = 6 | Inter-Day n = 9 | RSD% n = 6 | |
Taurine | 35.2978 ± 0.7821 | 2.22 | 2.35 | 2.42 | 2.71 |
Ginsenoside RG1 | 8.7050 ± 0.2457 | 2.82 | 4.69 | 4.30 | 3.24 |
Arenobufagin | 10.9163 ± 0.2826 | 2.59 | 3.13 | 3.95 | 4.33 |
TDCA | 43.8546 ± 1.8145 | 4.14 | 3.96 | 3.08 | 2.47 |
TCA | 29.7562 ± 1.3109 | 4.41 | 4.17 | 3.34 | 3.55 |
Bufalin | 6.9432 ± 0.2124 | 3.06 | 3.88 | 4.20 | 3.40 |
CA | 33.6313 ± 0.8419 | 2.50 | 3.52 | 3.58 | 2.58 |
Cinobufagin | 19.1693 ± 0.4315 | 2.25 | 2.71 | 2.63 | 3.91 |
Resibufogenin | 8.1053 ± 0.1211 | 1.49 | 2.88 | 3.00 | 3.00 |
Ginsenoside RG3 | 8.5907 ± 0.2642 | 3.08 | 2.63 | 2.60 | 2.25 |
CDCA | 14.7924 ± 0.5840 | 3.95 | 4.79 | 3.46 | 2.12 |
DCA | 5.7912 ± 0.1679 | 2.90 | 2.01 | 2.37 | 2.24 |
Tanshinone IIA | 0.0208 ± 0.0007 | 3.54 | 4.00 | 4.55 | 4.88 |
Analyte | Original (μg) | Added (μg) | Detected (μg) (±SD n = 3) | Recovery (%) (±SD n = 9) | RSD (%) |
---|---|---|---|---|---|
Taurine | 1764.89 | 2000.00 | 3734.82 ± 73.55 | 99.20 ± 3.00 | 3.02 |
1500.00 | 3263.21 ± 55.01 | ||||
1000.00 | 2756.97 ± 27.37 | ||||
Ginsenoside RG1 | 435.25 | 500.00 | 934.19 ± 22.09 | 98.86 ± 3.77 | 3.82 |
400.00 | 826.68 ± 12.69 | ||||
300.00 | 732.04 ± 14.85 | ||||
Arenobufagin | 545.82 | 900.00 | 1422.16 ± 4.83 | 99.28 ± 3.49 | 3.52 |
600.00 | 1156.98 ± 26.84 | ||||
300.00 | 841.64 ± 10.52 | ||||
TDCA | 2192.73 | 3000.00 | 5115.89 ± 43.01 | 98.64 ± 2.96 | 3.00 |
2000.00 | 4195.34 ± 57.22 | ||||
1000.00 | 3176.37 ± 43.66 | ||||
TCA | 1487.81 | 2000.00 | 3435.51 ± 54.39 | 99.26 ± 3.31 | 3.34 |
1500.00 | 2994.86 ± 70.89 | ||||
1000.00 | 2487.12 ± 24.57 | ||||
Bufalin | 347.16 | 500.00 | 843.70 ± 24.18 | 99.00 ± 4.18 | 4.23 |
400.00 | 744.40 ± 18.15 | ||||
300.00 | 642.24 ± 15.03 | ||||
CA | 1681.57 | 2000.00 | 3682.31 ± 57.38 | 99.20 ± 3.09 | 3.11 |
1500.00 | 3149.48 ± 52.01 | ||||
1000.00 | 2678.33 ± 37.17 | ||||
Cinobufagin | 958.47 | 1500.00 | 2428.88 ± 47.17 | 98.64 ± 3.38 | 3.00 |
1000.00 | 1963.89 ± 36.52 | ||||
500.00 | 1434.10 ± 3.47 | ||||
Resibufogenin | 405.27 | 500.00 | 896.21 ± 13.03 | 98.24 ± 3.20 | 3.25 |
400.00 | 795.04 ± 12.45 | ||||
300.00 | 702.54 ± 14.18 | ||||
Ginsenoside RG3 | 429.54 | 500.00 | 928.54 ± 19.34 | 99.63 ± 3.55 | 3.56 |
400.00 | 825.86 ± 17.65 | ||||
300.00 | 729.51 ± 11.68 | ||||
CDCA | 739.62 | 1500.00 | 2222.5 ± 54.47 | 98.67 ± 2.97 | 3.01 |
750.00 | 1475.11 ± 17.65 | ||||
500.00 | 1234.91 ± 19.78 | ||||
DCA | 289.56 | 600.00 | 888.35 ± 23.94 | 99.38 ± 3.23 | 3.25 |
300.00 | 584.70 ± 11.05 | ||||
150.00 | 439.51 ± 4.75 | ||||
Tanshinone IIA | 1.04 | 2.00 | 3.01 ± 0.09 | 101.02 ± 4.53 | 4.48 |
1.00 | 2.05 ± 0.05 | ||||
0.50 | 1.56 ± 0.03 |
2.5. Sample Analysis
Lot No. Compounds (mg/g) | Sample 1 | Sample 2 | Sample 3 | Sample 4 | Sample 5 | Sample 6 | Sample 7 | Sample 8 |
---|---|---|---|---|---|---|---|---|
Taurine | 13.8735 | 10.0161 | 15.1052 | 14.4826 | 13.6382 | 35.2978 | 24.4059 | 17.9399 |
Ginsenoside RG1 | 10.4003 | 4.7917 | 6.0041 | 5.4945 | 8.1709 | 8.7050 | 10.5062 | 9.8985 |
Arenobufagin | 6.5724 | 3.2283 | 3.9044 | 6.4789 | 4.8138 | 10.9163 | 7.2296 | 14.0551 |
TDCA | 43.2012 | 73.8723 | 52.7846 | 76.3674 | 96.1933 | 43.8546 | 78.2465 | 32.8261 |
TCA | 21.2145 | 21.3171 | 33.0645 | 44.2204 | 52.5620 | 29.7562 | 47.4044 | 21.5164 |
Bufalin | 4.3781 | 5.2646 | 2.7532 | 4.9919 | 3.4709 | 6.9432 | 5.4234 | 7.1698 |
CA | 13.2086 | 25.3780 | 15.5592 | 15.1331 | 16.1522 | 33.6313 | 23.6953 | 23.8216 |
Cinobufagin | 10.3166 | 6.1709 | 6.9125 | 11.3476 | 9.3716 | 19.1693 | 13.6489 | 15.7044 |
Resibufogenin | 5.0181 | 3.0739 | 3.1118 | 5.4689 | 4.1013 | 8.1053 | 6.3188 | 7.4538 |
Ginsenoside RG3 | 17.1353 | 3.2188 | 8.0643 | 8.0672 | 13.4715 | 8.5907 | 13.3079 | 6.4563 |
CDCA | 3.7939 | 4.6744 | 10.4556 | 6.6600 | 8.0945 | 14.7924 | 11.2672 | 5.8183 |
DCA | 5.5619 | 3.6310 | 2.2421 | 5.1843 | 6.1205 | 5.7912 | 10.0939 | 9.4447 |
Tanshinone IIA | 0.0026 | 0.0028 | 0.0207 | 0.0031 | 0.0134 | 0.0208 | 0.0239 | 0.0154 |
3. Experimental Section
3.1. Materials and Reagents
3.2. Preparation of Standard and Sample Solutions
3.3. Qualitative and Quantitative Analysis Conditions
3.3.1. HPLC Method for Qualitative Analysis
3.3.2. Q-TOF Method for Qualitative Analysis
3.3.3. UPLC-MS Method for Quantitative Analysis
Compounds | Rt (min) | Precursor Ion (m/z) | Daughter Ion (m/z) | Con Voltage (V) | Collision Energy (eV) |
---|---|---|---|---|---|
Taurine | 0.82 | 126.00 | 108.20 | 22 | 14 |
Ginsenoside Rg1 | 1.25 | 823.30 | 643.50 | 80 | 40 |
Arenobufagin | 2.23 | 417.20 | 399.10 | 45 | 30 |
TDCA | 3.39 | 522.28 | 486.61 | 50 | 25 |
TCA | 3.41 | 538.00 | 538.00 | 60 | 2 |
Astragaloside | 4.91 | 807.00 | 807.00 | 80 | 30 |
Bufalin | 5.22 | 387.30 | 351.20 | 35 | 22 |
CA | 5.41 | 391.30 | 355.30 | 0 | 20 |
Cinobufagin | 5.56 | 443.50 | 365.20 | 60 | 20 |
Resibufogenin | 5.59 | 385.30 | 367.20 | 35 | 18 |
Ginsenoside Rg3 | 5.75 | 807.00 | 365.00 | 90 | 5 |
CDCA | 6.13 | 357.60 | 161.20 | 40 | 20 |
DCA | 6.23 | 357.60 | 161.20 | 40 | 20 |
Tanshinone IIA | 7.28 | 295.10 | 277.00 | 40 | 20 |
3.4. Validation of Quantitative Method
3.4.1. Calibration Curve, LOD, and LOQ
3.4.2. Precision, Stability, Repeatability, and Recovery
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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- Sample Availability: Samples of the compounds (1) taurine, (2) ginsenoside Rg1, (3) arenobufagin, (4) TDCA, (5) TCA, (6) bufalin, (7) CA, (8) cinobufagin, (9) resibufogenin, (10) ginsenoside Rg3, (11) CDCA, (12) DCA, (13) tanshinone IIA are available from the authors.
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Chen, D.; Lin, S.; Xu, W.; Huang, M.; Chu, J.; Xiao, F.; Lin, J.; Peng, J. Qualitative and Quantitative Analysis of the Major Constituents in Shexiang Tongxin Dropping Pill by HPLC-Q-TOF-MS/MS and UPLC-QqQ-MS/MS. Molecules 2015, 20, 18597-18619. https://doi.org/10.3390/molecules201018597
Chen D, Lin S, Xu W, Huang M, Chu J, Xiao F, Lin J, Peng J. Qualitative and Quantitative Analysis of the Major Constituents in Shexiang Tongxin Dropping Pill by HPLC-Q-TOF-MS/MS and UPLC-QqQ-MS/MS. Molecules. 2015; 20(10):18597-18619. https://doi.org/10.3390/molecules201018597
Chicago/Turabian StyleChen, Daxin, Shan Lin, Wen Xu, Mingqing Huang, Jianfeng Chu, Fei Xiao, Jiumao Lin, and Jun Peng. 2015. "Qualitative and Quantitative Analysis of the Major Constituents in Shexiang Tongxin Dropping Pill by HPLC-Q-TOF-MS/MS and UPLC-QqQ-MS/MS" Molecules 20, no. 10: 18597-18619. https://doi.org/10.3390/molecules201018597