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Molecules 2015, 20(10), 18539-18550; doi:10.3390/molecules201018539

Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development

1
Biology Institute, Shandong Academy of Sciences, Jinan 250014, China
2
School of Food Science and Engineering, Qilu University of Technology, Jinan 250014, China
3
Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 32023, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 25 July 2015 / Revised: 10 September 2015 / Accepted: 25 September 2015 / Published: 12 October 2015
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [1840 KB, uploaded 12 October 2015]   |  

Abstract

In this study, we cloned the full-length cDNA of E-selectin of zebrafish (Danio rerio), analyzed its expression pattern and preliminarily explored its biological function. Zebrafish E-selectin cDNA is 3146 bp and encodes a putative 871 amino acid protein. All structural domains involved in E-selectin function are conserved in the putative protein. Whole-mount in situ hybridization of zebrafish at 24 and 48 h post-fertilization (hpf) revealed E-selectin expression mainly in vascular/endothelial progenitor cells in the posterior trunk and blood cells in the intermediate cell mass and posterior cardinal vein regions. Real-time quantitative RT-PCR analysis detected E-selectin expression at 0.2, 24 and 48 hpf and significantly decreased from 48 to 72 hpf. The expression of E-selectin, tumor necrosis factor-α and interleukin-1β was significantly upregulated at 22 to 72 h after induction with bacterial lipopolysaccharide. Thus, the structure of E-selectin protein is highly conserved among species, and E-selectin may be involved in embryonic development and essential for hematopoiesis and angiogenesis during embryonic development in zebrafish. Furthermore, we provide the first evidence of inflammatory mediators inducing E-selectin expression in non-mammalian vertebrates, which suggests that zebrafish E-selectin may be involved in inflammation and probably has similar biological function to mammalian E-selectin. View Full-Text
Keywords: zebrafish; E-selectin; identification; expression; lipopolysaccharide zebrafish; E-selectin; identification; expression; lipopolysaccharide
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Sun, G.; Liu, K.; Wang, X.; Liu, X.; He, Q.; Hsiao, C.-D. Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development. Molecules 2015, 20, 18539-18550.

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