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Molecules 2015, 20(1), 1543-1550; doi:10.3390/molecules20011543

Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species

1
National Agrobiodiversity Centre, National Academy of Agricultural Science, Rural Development Administration, Jeonju 560-500, Korea
2
Department of Biological and Environmental Science, Dongguk University, Seoul 100-175, Korea
3
Food Grain Policy Division, Ministry of Agriculture, Food and Rural Affairs, Sejong-si 339-012, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 6 October 2014 / Accepted: 14 January 2015 / Published: 16 January 2015
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [781 KB, uploaded 16 January 2015]   |  

Abstract

The temperate and herbaceous genus Vicia L. is a member of the legume tribe Fabeae of the subfamily Papilionoideae. The genus Vicia comprises 166 annual or perennial species distributed mainly in Europe, Asia, and North America, but also extending to the temperate regions of South America and tropical Africa. The use of simple sequence repeat (SSR) markers for Vicia species has not been investigated as extensively as for other crop species. In this study, we assessed the potential for cross-species amplification of cDNA microsatellite markers developed from common vetch (Vicia sativa subsp. sativa). For cross-species amplification of the SSRs, amplification was carried out with genomic DNA isolated from two to eight accessions of 22 different Vicia species. For individual species or subspecies, the transferability rates ranged from 33% for V. ervilia to 82% for V. sativa subsp. nigra with an average rate of 52.0%. Because the rate of successful SSR marker amplification generally correlates with genetic distance, these SSR markers are potentially useful for analyzing genetic relationships between or within Vicia species. View Full-Text
Keywords: cDNA-SSR; genetic diversity; transferability; Vicia L. cDNA-SSR; genetic diversity; transferability; Vicia L.
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Raveendar, S.; Lee, G.-A.; Jeon, Y.-A.; Lee, Y.J.; Lee, J.-R.; Cho, G.-T.; Cho, J.-H.; Park, J.-H.; Ma, K.-H.; Chung, J.-W. Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species. Molecules 2015, 20, 1543-1550.

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