Next Article in Journal
Accumulation of Astragalosides and Related Gene Expression in Different Organs of Astragalus Membranaceus Bge. var Mongholicus (Bge.)
Previous Article in Journal
ROS-Dependent Antiproliferative Effect of Brassinin Derivative Homobrassinin in Human Colorectal Cancer Caco2 Cells
Article Menu

Export Article

Open AccessArticle
Molecules 2014, 19(8), 10898-10921; doi:10.3390/molecules190810898

Dereplication Guided Discovery of Secondary Metabolites of Mixed Biosynthetic Origin from Aspergillus aculeatus

1
Chemodiversity Group, Department of Systems Biology, Søltofts Plads B221, Technical University of Denmark, Kgs. Lyngby DK-2800, Denmark
2
Department of Chemistry, Kemitorvet B201, Technical University of Denmark, Kgs. Lyngby DK-2800, Denmark
*
Author to whom correspondence should be addressed.
Received: 16 June 2014 / Revised: 15 July 2014 / Accepted: 16 July 2014 / Published: 25 July 2014
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [2483 KB, uploaded 30 July 2014]   |  

Abstract

Investigation of the chemical profile of the industrially important black filamentous fungus Aspergillus aculeatus by UHPLC-DAD-HRMS and subsequent dereplication has led to the discovery of several novel compounds. Isolation and extensive 1D and 2D NMR spectroscopic analyses allowed for structural elucidation of a dioxomorpholine, a unique okaramine, an aflavinine and three novel structures of mixed biosynthetic origin, which we have named aculenes A–C. Moreover, known analogues of calbistrins, okaramines and secalonic acids were detected. All novel compounds were tested for antifungal activity against Candida albicans, however all showed only weak or no activity. Aspergillus aculeatus IBT 21030 was additionally shown to be capable of producing sclerotia. Examination of the sclerotia revealed a highly regulated production of metabolites in these morphological structures. View Full-Text
Keywords: Aspergillus aculeatus; Aspergilli; natural products; secondary metabolism; dereplication; sclerotia Aspergillus aculeatus; Aspergilli; natural products; secondary metabolism; dereplication; sclerotia
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Petersen, L.M.; Hoeck, C.; Frisvad, J.C.; Gotfredsen, C.H.; Larsen, T.O. Dereplication Guided Discovery of Secondary Metabolites of Mixed Biosynthetic Origin from Aspergillus aculeatus. Molecules 2014, 19, 10898-10921.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top