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Molecules 2014, 19(7), 9051-9069; doi:10.3390/molecules19079051

Targeted Lipid Analysis of Haemolytic Mycelial Extracts of Aspergillus niger

1
Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia
2
National Institute of Chemistry, 1000 Ljubljana, Slovenia
3
Department of Molecular and Biomedical Sciences, Jožef Stefan Institute, 1000 Ljubljana, Slovenia
4
Department of Chemistry and Biochemistry, Faculty of Chemistry and Chemical Technology, University of Ljubljana, 1000 Ljubljana, Slovenia
5
Department of Physics, Bioorganic Chemistry Laboratory, University of Trento, Via Sommarive 14, I-38123 Povo-Trento, Italy
6
Biophysical Institute, CNR, via alla Cascata 56/C, I-38123 Povo, Trento, Italy
*
Author to whom correspondence should be addressed.
Received: 28 February 2014 / Revised: 19 June 2014 / Accepted: 23 June 2014 / Published: 30 June 2014
(This article belongs to the Special Issue Fatty Acids)
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Abstract

Ethanolic extracts of mycelia from Aspergillus niger (strain N402) grown in liquid media were observed to have haemolytic activity on bovine erythrocytes. This haemolytic activity decreased significantly during the time of growth (1–3 days). Moreover, when A. niger was grown on carbon-deprived medium, the efficiency of this haemolytic activity in the ethanolic extracts was much lower than when grown in carbon-enriched medium, and became almost undetectable after 3 days of growth in carbon-deprived medium. The lipid composition of these ethanolic extracts was analysed by liquid chromatography–electrospray ionisation tandem mass spectrometry. This haemolytic activity can be mainly linked to the relative levels of the molar ratios of the unsaturated fatty acids and lysophosphatidylcholines. View Full-Text
Keywords: Aspergillus niger; haemolytic activity; fatty acids; lipid composition; liquid chromatography-selectrospray ionisation tandem mass spectrometry Aspergillus niger; haemolytic activity; fatty acids; lipid composition; liquid chromatography-selectrospray ionisation tandem mass spectrometry
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Novak, M.; Sepčić, K.; Kraševec, N.; Križaj, I.; Maček, P.; Anderluh, G.; Guella, G.; Mancini, I. Targeted Lipid Analysis of Haemolytic Mycelial Extracts of Aspergillus niger. Molecules 2014, 19, 9051-9069.

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