In Vitro and in Vivo Antitumor Activity of Scutebarbatine A on Human Lung Carcinoma A549 Cell Lines
Abstract
:1. Introduction
2. Results and Discussion
2.1. SBT-A Inhibited the Proliferation of A549 Cells in Vitro
2.2. Effect of SBT-A on the Morphological Changes of A549 Cells
2.3. Flow Cytometry Assay of A549 Apoptosis Induced by SBT-A
2.4. Effect of SBT-A on the Expressions of Caspase Family and Bcl-2 Family Proteins
2.5. In Vivo Antitumor Efficacy Study
3. Experimental
3.1. Chemicals
3.2. Animals
3.3. Cells Culture and MTT Assay
3.4. Apoptosis Assay by Hoechst 33258
3.5. Determination of Cell Apoptosis by Annexin V/PI Double Staining
3.6. Western Blot Analysis
3.7. Antitumor Activity Study of SBT-A in Vivo
3.8. Statistical Analysis
4. Conclusions
Author Contributions
Conflicts of Interest
References
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- Sample Availability: Not available.
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Yang, X.-K.; Xu, M.-Y.; Xu, G.-S.; Zhang, Y.-L.; Xu, Z.-X. In Vitro and in Vivo Antitumor Activity of Scutebarbatine A on Human Lung Carcinoma A549 Cell Lines. Molecules 2014, 19, 8740-8751. https://doi.org/10.3390/molecules19078740
Yang X-K, Xu M-Y, Xu G-S, Zhang Y-L, Xu Z-X. In Vitro and in Vivo Antitumor Activity of Scutebarbatine A on Human Lung Carcinoma A549 Cell Lines. Molecules. 2014; 19(7):8740-8751. https://doi.org/10.3390/molecules19078740
Chicago/Turabian StyleYang, Xiao-Kun, Ming-Yuan Xu, Gui-Sen Xu, Yu-Lan Zhang, and Zhao-Xia Xu. 2014. "In Vitro and in Vivo Antitumor Activity of Scutebarbatine A on Human Lung Carcinoma A549 Cell Lines" Molecules 19, no. 7: 8740-8751. https://doi.org/10.3390/molecules19078740