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Molecules 2014, 19(6), 7516-7527; doi:10.3390/molecules19067516
Article

Sesamin Enhances Cholesterol Efflux in RAW264.7 Macrophages

1
, 2
, 3
, 3
 and 2,*
Received: 14 April 2014; in revised form: 29 May 2014 / Accepted: 30 May 2014 / Published: 6 June 2014
(This article belongs to the Section Metabolites)
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Abstract: Foam cells formation as a result of the uncontrolled cytophagy of modified cholesterol by macrophages plays a key role in the occurrence and development of atherosclerosis. Sesamin is an active constituent of Sesamum indicum which has been shown to possess multiple pharmacological activities. In this work, we investigated the effects of sesamin on foam cell formation and cholesterol efflux in RAW264.7 macrophages. Sesamin dose-dependently inhibited the enhanced cholesterol accumulation elicited by oxidized low-density lipoprotein cholesterol (oxLDL) in RAW264.7 cells. Treatment with sesamin (10 μM) significantly enhanced cholesterol efflux mediated by high-density lipoprotein (HDL). Realtime quantitative PCR and luciferase assays showed that sesamin significantly increased the mRNA levels of PPARγ, LXRα, and ABCG1, and increased the transcriptional activity of PPARγ. The stimulating effect of sesamin on cholesterol efflux was substantially inhibited by the co-treatment with GW9662, a potent inhibitor of PPARγ. These results suggest that sesamin is a new inhibitor of foam cell formation that may stimulate cholesterol efflux through upregulation of the PPARγ-LXRα-ABCG1 pathway.
Keywords: sesamin; atherosclerosis; foam cell; cholesterol efflux; macrophage sesamin; atherosclerosis; foam cell; cholesterol efflux; macrophage
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Liu, N.; Wu, C.; Sun, L.; Zheng, J.; Guo, P. Sesamin Enhances Cholesterol Efflux in RAW264.7 Macrophages. Molecules 2014, 19, 7516-7527.

AMA Style

Liu N, Wu C, Sun L, Zheng J, Guo P. Sesamin Enhances Cholesterol Efflux in RAW264.7 Macrophages. Molecules. 2014; 19(6):7516-7527.

Chicago/Turabian Style

Liu, Nan; Wu, Chongming; Sun, Lizhong; Zheng, Jun; Guo, Peng. 2014. "Sesamin Enhances Cholesterol Efflux in RAW264.7 Macrophages." Molecules 19, no. 6: 7516-7527.



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