Sesamin Enhances Cholesterol Efflux in RAW264.7 Macrophages
Abstract
:1. Introduction
2. Results and Discussion
2.1. Sesamin Inhibits oxLDL-Induced Cholesterol Accumulation in RAW264.7 Cells
2.2. Sesamin Enhances Cholesterol Efflux from RAW264.7 Macrophages
2.3. Sesamin Increases Transcription Of Cholesterol Efflux-Associated Genes
2.4. The Cholesterol Efflux-Promoting Effect of Sesamin is Abolished by Pparγ Antagonist Gw9662
2.5. Sesamin Shows no Effect on Oxldl Uptake in Raw264.7 Macrophages
3. Experimental
3.1. Materials
3.2. Cell Culture and Cholesterol Accumulation Assay
3.3. Cellular Cholesterol Efflux Experiments
3.4. Quantitative Real-Time PCR
Name | Forward (5'–3') | Reverse (5'–3') |
---|---|---|
PPARγ | GCAGCTACTGCATGTGATCAAGA | GTCAGCGGGTGGGACTTTC |
LXRα | AGGAGTGTCGACTTCGCAAA | CTCTTCTTGCCGCTTCAGTTT |
ABCA1 | CCCAGAGCAAAAAGGGACTC | GGTCATCATCACTTTGGTCCTTG |
ABCG1 | CAAGACCCTTTTGAAAGGGATCTC | GCCAGAATATTCATGAGTGTGGAC |
CD36 | CAAGCTCCTTGGCATGGTAGA | TGGATTTGCAAGCACAATATGAA |
SR-1 | TTAAAGGTGATCGGGGACAAA | CAACCAGTCGAACTGTCTTAAG |
SR-2 | TTAAAGGTGATCGGGGACAAA | AGCTGATCTTAAAAGGGTCTTG |
β-actin | CCTGGCACCCAGCACAAT | GCCGATCCACACACGGAGTACT |
3.5. 25-NBD Cholesterol Uptake Assay
3.6. Luciferase Assay
3.7. Statistics Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Liu, N.; Wu, C.; Sun, L.; Zheng, J.; Guo, P. Sesamin Enhances Cholesterol Efflux in RAW264.7 Macrophages. Molecules 2014, 19, 7516-7527. https://doi.org/10.3390/molecules19067516
Liu N, Wu C, Sun L, Zheng J, Guo P. Sesamin Enhances Cholesterol Efflux in RAW264.7 Macrophages. Molecules. 2014; 19(6):7516-7527. https://doi.org/10.3390/molecules19067516
Chicago/Turabian StyleLiu, Nan, Chongming Wu, Lizhong Sun, Jun Zheng, and Peng Guo. 2014. "Sesamin Enhances Cholesterol Efflux in RAW264.7 Macrophages" Molecules 19, no. 6: 7516-7527. https://doi.org/10.3390/molecules19067516