Abstract: We studied the expression of the non-metastatic clone 23 type 1 (nm23H1) gene, vascular endothelial growth factor (VEGF)-C, and its receptor VEGFR-3 using an in situ hybridization technique and immunohistochemical analyses with prostate cancer tissues and adjacent benign tissues of 52 human archival cases. The association between VEGF-C expression, microlymphatic count (MLC), and staining intensity for nm23H1 and VEGFR-3 was used to evaluate tumor metastasis and survival rate. MLC values were significantly higher in tumorous tissue than in non-cancerous tissue. VEGF-C mRNA, VEGFR-3, and nm23H1 were highly expressed in tumorous tissue. VEGFR-3 expression was greater in VEGF-C mRNA-positive tumors than in VEGF-C mRNA-negative tumors. The association of VEGFR-3 expression with VEGF-C mRNA and MLC suggested that the poor prognosis and tumor metastasis associated with VEGFR-3 expression may be due, in part, to its role in promoting angiogenesis. VEGF-C expression was significantly associated with tumor lymphangiogenesis, angiogenesis, and immune response as a potent multifunctional stimulating factor in prostate cancer. Expression of nm23H1 was significantly inversely correlated with lymph node metastasis. Furthermore, there was a strong negative correlation between the expression of nm23H1, VEGF-C mRNA, and MLC. These findings provide important information for prophylactic, diagnostic, and therapeutic strategies for prostate cancer.
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Yang, Z.-S.; Xu, Y.-F.; Huang, F.-F.; Ding, G.-F. Associations of nm23H1, VEGF-C, and VEGF-3 Receptor in Human Prostate Cancer. Molecules 2014, 19, 6851-6862.
Yang Z-S, Xu Y-F, Huang F-F, Ding G-F. Associations of nm23H1, VEGF-C, and VEGF-3 Receptor in Human Prostate Cancer. Molecules. 2014; 19(5):6851-6862.
Yang, Zui-Su; Xu, Yin-Feng; Huang, Fang-Fang; Ding, Guo-Fang. 2014. "Associations of nm23H1, VEGF-C, and VEGF-3 Receptor in Human Prostate Cancer." Molecules 19, no. 5: 6851-6862.