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Molecules 2014, 19(5), 6838-6850; doi:10.3390/molecules19056838
Communication

A New Diterpene from Litsea cubeba Fruits: Structure Elucidation and Capability to Induce Apoptosis in HeLa Cells

1,* , 1, 1 and 1,2,*
Received: 14 April 2014; in revised form: 16 May 2014 / Accepted: 21 May 2014 / Published: 23 May 2014
(This article belongs to the Section Metabolites)
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Abstract: A new diterpene, identified as (+)-6-(4-hydroxy-4-methyl-2-pentenoyl)-4,6-dimethyl-5-(3-methyl-2-butenyl)-1,3-cyclohexadienecarbaldehyde (1, cubelin), was isolated from a methanol extract of Litsea cubeba fruits by normal phase column chromatography and purified by preparative HPLC. The structure elucidation was conducted by spectroscopic methods (UV, IR, ESI-TOF-MS, 1-D and 2-D NMR). Cubelin exhibited activity against HeLa cell viability and proliferation. The cells also exhibited changes in nuclear morphology which are hallmarks of apoptotic cell death. The presence of cleaved caspase-3/-7, caspase-8 and caspase-9 in the cubelin treated population indicated the potential of the compound to induce apoptosis in HeLa cells via both intrinsic and extrinsic pathways.
Keywords: Litsea cubeba; cubelin; apoptosis; HeLa cell; caspase Litsea cubeba; cubelin; apoptosis; HeLa cell; caspase
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Trisonthi, P.; Sato, A.; Nishiwaki, H.; Tamura, H. A New Diterpene from Litsea cubeba Fruits: Structure Elucidation and Capability to Induce Apoptosis in HeLa Cells. Molecules 2014, 19, 6838-6850.

AMA Style

Trisonthi P, Sato A, Nishiwaki H, Tamura H. A New Diterpene from Litsea cubeba Fruits: Structure Elucidation and Capability to Induce Apoptosis in HeLa Cells. Molecules. 2014; 19(5):6838-6850.

Chicago/Turabian Style

Trisonthi, Piyapat; Sato, Akihiko; Nishiwaki, Hisashi; Tamura, Hirotoshi. 2014. "A New Diterpene from Litsea cubeba Fruits: Structure Elucidation and Capability to Induce Apoptosis in HeLa Cells." Molecules 19, no. 5: 6838-6850.


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