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Molecules 2014, 19(5), 6504-6523; doi:10.3390/molecules19056504
Article

Evaluation of Antioxidant Activity, Polyphenolic Compounds, Amino Acids and Mineral Elements of Representative Genotypes of Lonicera edulis

1,2,* , 3
, 4,5
, 6
, 1
, 1
, 3
, 2
, 7
, 8
, 9
 and 10
1 Department of Viticulture and Enology, Faculty of Horticulture, Mendel University, Valticka 337, CZ-691 44 Lednice, Czech Republic 2 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Zemedelska 1, CZ-613 00 Brno, Czech Republic 3 Institute for Teacher Training, Faculty of Central European Studies, Constantine the Philosopher University, Drazovska 4, SK-949 74 Nitra, Slovak Republic 4 Faculty of Military Health Sciences, University of Defence, Trebesska 1575, CZ-500 01 Hradec Kralove, Czech Republic 5 Karel English College, Sujanovo namesti 356/1, 60200 Brno, Czech Republic 6 Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Kolejni 4, CZ-612 00 Brno, Czech Republic 7 Department of Vegetable Science and Floriculture, Faculty of Horticulture, Mendel University, Lednice, Valtická 337, CZ-691 44 Lednice, Czech Republic 8 Department of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University, Namesti T.G.Masaryka 275, 762 72 Zlin, Czech Republic 9 Department of Garden and Landscape Architecture, Faculty of Horticulture, Mendel University, Valticka 337, CZ-691 44 Lednice, Czech Republic 10 Department of Applied Pharmacy, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho 1, CZ-612 42 Brno, Czech Republic
* Author to whom correspondence should be addressed.
Received: 26 January 2014 / Revised: 16 May 2014 / Accepted: 16 May 2014 / Published: 21 May 2014
(This article belongs to the collection Bioactive Compounds)
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Abstract

The aim of this study was to evaluate the bioactive substances in 19 berry cultivars of edible honeysuckle (Lonicera edulis). A statistical evaluation was used to determine the relationship between the content of selected bioactive substances and individual cultivars. Regarding mineral elements, the content of sodium was measured using potentiometry and spectrophotometry. The content of selected polyphenolic compounds with high antioxidant activity was determined by a HPLC–UV/ED method. The total amount of polyphenols was determined by the Folin-Ciocalteu method. The antioxidant activity was determined using five methods (DPPH, FRAP, ABTS, FR and DMPD) that differ in their principles. The content of 13 amino acids was determined by ion-exchange chromatography. The experimental results obtained for the different cultivars were evaluated and compared by statistical and bioinformatic methods. A unique feature of this study lies in the exhaustive analysis of the chosen parameters (amino acids, mineral elements, polyphenolic compounds and antioxidant activity) during one growing season.
Keywords: Lonicera edulis; mineral compounds; phenolic compounds; antioxidant activity; amino acids; bioinformatics analysis Lonicera edulis; mineral compounds; phenolic compounds; antioxidant activity; amino acids; bioinformatics analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sochor, J.; Jurikova, T.; Pohanka, M.; Skutkova, H.; Baron, M.; Tomaskova, L.; Balla, S.; Klejdus, B.; Pokluda, R.; Mlcek, J.; Trojakova, Z.; Saloun, J. Evaluation of Antioxidant Activity, Polyphenolic Compounds, Amino Acids and Mineral Elements of Representative Genotypes of Lonicera edulis. Molecules 2014, 19, 6504-6523.

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