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Molecules 2014, 19(5), 6504-6523; doi:10.3390/molecules19056504
Article

Evaluation of Antioxidant Activity, Polyphenolic Compounds, Amino Acids and Mineral Elements of Representative Genotypes of Lonicera edulis

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Received: 26 January 2014 / Revised: 16 May 2014 / Accepted: 16 May 2014 / Published: 21 May 2014
(This article belongs to the collection Bioactive Compounds)
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Abstract

The aim of this study was to evaluate the bioactive substances in 19 berry cultivars of edible honeysuckle (Lonicera edulis). A statistical evaluation was used to determine the relationship between the content of selected bioactive substances and individual cultivars. Regarding mineral elements, the content of sodium was measured using potentiometry and spectrophotometry. The content of selected polyphenolic compounds with high antioxidant activity was determined by a HPLC–UV/ED method. The total amount of polyphenols was determined by the Folin-Ciocalteu method. The antioxidant activity was determined using five methods (DPPH, FRAP, ABTS, FR and DMPD) that differ in their principles. The content of 13 amino acids was determined by ion-exchange chromatography. The experimental results obtained for the different cultivars were evaluated and compared by statistical and bioinformatic methods. A unique feature of this study lies in the exhaustive analysis of the chosen parameters (amino acids, mineral elements, polyphenolic compounds and antioxidant activity) during one growing season.
Keywords: Lonicera edulis; mineral compounds; phenolic compounds; antioxidant activity; amino acids; bioinformatics analysis Lonicera edulis; mineral compounds; phenolic compounds; antioxidant activity; amino acids; bioinformatics analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sochor, J.; Jurikova, T.; Pohanka, M.; Skutkova, H.; Baron, M.; Tomaskova, L.; Balla, S.; Klejdus, B.; Pokluda, R.; Mlcek, J.; Trojakova, Z.; Saloun, J. Evaluation of Antioxidant Activity, Polyphenolic Compounds, Amino Acids and Mineral Elements of Representative Genotypes of Lonicera edulis. Molecules 2014, 19, 6504-6523.

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