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Molecules 2014, 19(5), 6123-6141; doi:10.3390/molecules19056123

Distribution of Nucleosides in Populations of Cordyceps cicadae

1,* , 2
1 Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, Yunnan, China 2 Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan, China 3 Department of Biological Sciences, College of Engineering, Forestry and Natural Science, Northern Arizona University, Flagstaff, AZ 86011-5640, USA
* Author to whom correspondence should be addressed.
Received: 15 January 2014 / Revised: 25 April 2014 / Accepted: 5 May 2014 / Published: 14 May 2014
(This article belongs to the Section Metabolites)
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A rapid HPLC method had been developed and used for the simultaneous determination of 10 nucleosides (uracil, uridine, 2'-deoxyuridine, inosine, guanosine, thymidine, adenine, adenosine, 2'-deoxyadenosine and cordycepin) in 10 populations of Cordyceps cicadae, in order to compare four populations of Ophicordyceps sinensis and one population of Cordyceps militaris. Statistical analysis system (SAS) 8.1 was used to analyze the nucleoside data. The pattern of nucleoside distribution was analyzed in the sampled populations of C. cicadae, O. sinensis and C. militaris, using descriptive statistical analysis, nested analysis and Q cluster analysis. The total amount of the 10 nucleosides in coremium was 1,463.89–5,678.21 µg/g in 10 populations of C. cicadae, 1,369.80–3,941.64 µg/g in sclerotium. The average contents of the 10 analytes were 4,392.37 µg/g and 3,016.06 µg/g in coremium and sclerotium, respectively. The coefficient of variation (CV) of nucleosides ranged from 8.36% to 112.36% in coremium of C. cicadae, and from 10.77% to 155.87% in sclerotium of C. cicadae. The CV of the nucleosides was wide within C. cicadae populations. The nested variation analysis by the nine nucleosides’ distribution indicated that about 42.29% of the nucleoside variability in coremium was attributable to the differentiation among populations, and the remaining 57.71% resided in the populations. It was also shown that about 28.94% of the variation in sclerotium was expressed between populations, while most of the variation (71.06%) corresponded to the populations.
Keywords: Cordyceps cicadae; nucleosides; distribution Cordyceps cicadae; nucleosides; distribution
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Zeng, W.-B.; Yu, H.; Ge, F.; Yang, J.-Y.; Chen, Z.-H.; Wang, Y.-B.; Dai, Y.-D.; Adams, A. Distribution of Nucleosides in Populations of Cordyceps cicadae. Molecules 2014, 19, 6123-6141.

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