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Molecules 2014, 19(5), 6080-6105; doi:10.3390/molecules19056080

Issues and Prospects of microRNA-Based Biomarkers in Blood and Other Body Fluids

1, 1, 3, 2,*  and 1,*
1 Institute for Systems Biology, Seattle, WA 98109, USA 2 Institute of Medical Systems Biology, Guangdong Medical College, Dongguan, Guangdong 523808, China 3 Laboratory of the Biology of Addictive Diseases, Rockefeller University, New York, NY 10065, USA
* Authors to whom correspondence should be addressed.
Received: 17 March 2014 / Revised: 7 May 2014 / Accepted: 12 May 2014 / Published: 14 May 2014
(This article belongs to the Special Issue miRNAs as Probes to Monitor Cancer and Neurodegenerative Disorders)
View Full-Text   |   Download PDF [271 KB, 18 June 2014; original version 18 June 2014]


Cell-free circulating microRNAs (miRNAs) in the blood are good diagnostic biomarker candidates for various physiopathological conditions, including cancer, neurodegeneration, diabetes and other diseases. Since their discovery in 2008 as blood biomarkers, the field has expanded rapidly with a number of important findings. Despite the initial optimistic views of their potential for clinical application, there are currently no circulating miRNA-based diagnostics in use. In this article, we review the status of circulating miRNAs, examine different analytical approaches, and address some of the challenges and opportunities.
Keywords: diagnostic; exosomes; microvesicles; quantitative methods diagnostic; exosomes; microvesicles; quantitative methods
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chevillet, J.R.; Lee, I.; Briggs, H.A.; He, Y.; Wang, K. Issues and Prospects of microRNA-Based Biomarkers in Blood and Other Body Fluids. Molecules 2014, 19, 6080-6105.

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