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Molecules 2014, 19(2), 1939-1955; doi:10.3390/molecules19021939

Use of EST-SSR Markers for Evaluating Genetic Diversity and Fingerprinting Celery (Apium graveolens L.) Cultivars

Department of Vegetable Science, College of Agriculture and Biotechnology, China Agricultural University, No.2 Yuanmingyuan Xi Lu, Haidian District, Beijing 100193, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 23 December 2013 / Revised: 5 February 2014 / Accepted: 7 February 2014 / Published: 10 February 2014
(This article belongs to the Section Molecular Diversity)
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Celery (Apium graveolens L.) is one of the most economically important vegetables worldwide, but genetic and genomic resources supporting celery molecular breeding are quite limited, thus few studies on celery have been conducted so far. In this study we made use of simple sequence repeat (SSR) markers generated from previous celery transcriptome sequencing and attempted to detect the genetic diversity and relationships of commonly used celery accessions and explore the efficiency of the primers used for cultivars identification. Analysis of molecular variance (AMOVA) of Apium graveolens L. var. dulce showed that approximately 43% of genetic diversity was within accessions, 45% among accessions, and 22% among horticultural types. The neighbor-joining tree generated by unweighted pair group method with arithmetic mean (UPGMA), and population structure analysis, as well as principal components analysis (PCA), separated the cultivars into clusters corresponding to the geographical areas where they originated. Genetic distance analysis suggested that genetic variation within Apium graveolens was quite limited. Genotypic diversity showed any combinations of 55 genic SSRs were able to distinguish the genotypes of all 30 accessions.
Keywords: celery; EST-SSR; genetic diversity; fingerprint; genotypic diversity celery; EST-SSR; genetic diversity; fingerprint; genotypic diversity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Fu, N.; Wang, P.-Y.; Liu, X.-D.; Shen, H.-L. Use of EST-SSR Markers for Evaluating Genetic Diversity and Fingerprinting Celery (Apium graveolens L.) Cultivars. Molecules 2014, 19, 1939-1955.

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