Absorbability, Mechanism and Structure-Property Relationship of Three Phenolic Acids from the Flowers of Trollius chinensis
Abstract
:1. Introduction
2. Results and Discussion
2.1. Results
2.1.1. Validation of HPLC Analytical Method
2.1.2. Validation of Caco-2 Monolayer Model
2.1.3. Non-Toxic Dose Range of Test Compounds
Compounds | Survival Rate: % | ||
---|---|---|---|
25 μmol/L | 50 μmol/L | 100 μmol/L | |
PA | 118.8 ± 11.6 | 110.5 ± 3.8 | 124.5 ± 2.2 |
GA | 107.1 ± 5.7 | 118.9 ± 13.7 | 123.6 ± 6.2 |
TS | 98.9 ± 4.3 | 107.3 ± 7.0 | 118.2 ± 8.1 |
Paclitaxel | 67.3 ± 8.2 | 45.5 ± 6.7 | 37.4 ± 3.5 |
2.1.4. Transport of Test Compounds
Concentration (μmol/L) | Papp AP→BL (×10−5 cm/s) | Papp BL→AP (×10−5 cm/s) | Pratio (Papp AP→BL/Papp BL→AP) | |
---|---|---|---|---|
PA | 25 | 3.17 ± 0.04 | 2.54 ± 0.22 | 1.25 |
50 | 2.80 ± 0.13 | 2.56 ± 0.19 | 1.1 | |
100 | 2.96 ± 0.11 | 2.59 ± 0.14 | 1.14 | |
average | 2.98 ± 0.13 | 2.56 ± 0.18 | 1.16 | |
GA | 25 | 2.14 ± 0.20 | 2.21 ± 0.08 | 0.96 |
50 | 1.59 ± 0.02 | 2.00 ± 0.07 | 0.8 | |
100 | 1.70 ± 0.12 | 1.94 ± 0.10 | 0.88 | |
average | 1.81 ± 0.11 | 2.05 ± 0.08 | 0.88 | |
TS | 25 | 0.86 ± 0.16 | 0.86 ± 0.04 | 1 |
50 | 1.06 ± 0.03 | 1.08 ± 0.06 | 0.98 | |
100 | 0.77 ± 0.09 | 0.80 ± 0.03 | 0.96 | |
average | 0.89 ± 0.09 | 0.91 ± 0.04 | 0.98 |
2.1.5. Time Course
2.1.6. Concentration Dependence
2.1.7. Molecular Properties
Compound | Molecular Mass | LogP | TPSA | Hd | Hr | R | V |
---|---|---|---|---|---|---|---|
PA | 236.26 | 2.67 | 66.761 | 2 | 4 | 4 | 0 |
GA | 236.26 | 2.27 | 55.767 | 1 | 4 | 2 | 0 |
TS | 398.4 | 0.83 | 145.913 | 5 | 9 | 7 | 0 |
2.2. Discussion
3. Experimental
3.1. General
3.2. Plant Material
3.3. Extraction and Isolation of GA, PA and TS
3.4. Cell Culture
3.5. MTT Assay
3.6. Cell Differentiation
3.7. Transport Experiment
3.8. HPLC Analysis
3.8.1. Chromatographic Condition
3.8.2. Linearity, Precision and Stability
3.9. Calculation of Molecular Properties
3.10. Data Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Wu, X.-W.; Wang, R.-F.; Liu, L.-J.; Guo, L.-N.; Zhao, C. Absorbability, Mechanism and Structure-Property Relationship of Three Phenolic Acids from the Flowers of Trollius chinensis. Molecules 2014, 19, 18129-18138. https://doi.org/10.3390/molecules191118129
Wu X-W, Wang R-F, Liu L-J, Guo L-N, Zhao C. Absorbability, Mechanism and Structure-Property Relationship of Three Phenolic Acids from the Flowers of Trollius chinensis. Molecules. 2014; 19(11):18129-18138. https://doi.org/10.3390/molecules191118129
Chicago/Turabian StyleWu, Xiu-Wen, Ru-Feng Wang, Li-Jia Liu, Li-Na Guo, and Can Zhao. 2014. "Absorbability, Mechanism and Structure-Property Relationship of Three Phenolic Acids from the Flowers of Trollius chinensis" Molecules 19, no. 11: 18129-18138. https://doi.org/10.3390/molecules191118129