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Molecules 2014, 19(11), 17345-17355; doi:10.3390/molecules191117345

Bioconversion of Rebaudioside I from Rebaudioside A

1
The Coca-Cola Company, Atlanta, GA 30313, USA
2
AMRI-Albany, Analytical Development, Albany, NY 12212, USA
3
PureCircle Limited, Lengkuk Teknologi, Bandar Enstek 71760, Malaysia
4
Libragen SA, 3 Rue des Satellites, Toulouse 31400, France
*
Author to whom correspondence should be addressed.
Received: 22 August 2014 / Revised: 3 October 2014 / Accepted: 23 October 2014 / Published: 28 October 2014
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [458 KB, uploaded 28 October 2014]   |  

Abstract

To supply the increasing demand of natural high potency sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of rebaudioside A to rebaudioside I using a glucosyltransferase enzyme. This bioconversion reaction adds one sugar unit with a 1→3 linkage. We utilized 1D and 2D NMR spectroscopy (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D TOCSY and NOESY) and mass spectral data to fully characterize rebaudioside I. View Full-Text
Keywords: Stevia rebaudiana; structure characterization; bioconversion; rebaudioside I; rebaudioside A Stevia rebaudiana; structure characterization; bioconversion; rebaudioside I; rebaudioside A
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Prakash, I.; Bunders, C.; Devkota, K.P.; Charan, R.D.; Ramirez, C.; Snyder, T.M.; Priedemann, C.; Markosyan, A.; Jarrin, C.; Halle, R.T. Bioconversion of Rebaudioside I from Rebaudioside A. Molecules 2014, 19, 17345-17355.

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