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Molecules 2014, 19(10), 16158-16178; doi:10.3390/molecules191016158

Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-Reperfusion

1
Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, China
2
School of Information Technology, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, China
3
School of Acupuncture and Tuina, Chengdu University of Traditional Chinese Medicine, No.1166, Liutai Avenue, Wenjiang District, Chengdu 611137, Sichuan, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 21 June 2014 / Revised: 12 September 2014 / Accepted: 15 September 2014 / Published: 9 October 2014
(This article belongs to the Section Molecular Diversity)
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Abstract

This study investigated genome-wide gene expressions and the cardioprotective effects of electro-acupuncture pretreatment at the PC6 Neiguan acupoint on myocardial ischemia reperfusion (I/R) injury. Male SD rats were randomly divided into four groups: sham operation (SO), I/R, electro-acupuncture at the PC6 Neiguan acupoint pretreatment (EA) and electro-acupuncture at non-acupoint pretreatment (NA). Compared with the I/R group, the survival rate of the EA group was significantly increased, the arrhythmia score, infarction area, serum concentrations of CK, LDH and CK-Mb and plasma level of cTnT were significantly decreased. RNA-seq results showed that 725 genes were up-regulated and 861 genes were down-regulated under I/R conditions compared to the SO group; both EA and NA reversed some of these gene expression levels (592 in EA and 238 in NA group). KEGG pathway analysis indicated that these genes were involved in multiple pathways, including ECM, MAPK signaling, apoptosis, cytokine and leukocyte pathways. In addition, some pathways were uniquely regulated by EA, but not NA pretreatment, such as oxidative stress, cardiac muscle contraction, gap junction, vascular smooth muscle contraction, hypertrophic, NOD-like receptor, and P53 and B-cell receptor pathways. This study was first to reveal the gene expression signatures of acute myocardial I/R injury and electro-acupuncture pretreatment in rats. View Full-Text
Keywords: electro-acupuncture pretreatment; myocardial ischemia-reperfusion; RNA-seq; gene expression profiling; pathway electro-acupuncture pretreatment; myocardial ischemia-reperfusion; RNA-seq; gene expression profiling; pathway
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Huang, Y.; Lu, S.-F.; Hu, C.-J.; Fu, S.-P.; Shen, W.-X.; Liu, W.-X.; Li, Q.; Wang, N.; He, S.-Y.; Liang, F.-R.; Zhu, B.-M. Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-Reperfusion. Molecules 2014, 19, 16158-16178.

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