- freely available
Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp
AbstractThe solution properties of four fractions (LPI–IV) from crude longan pulp polysaccharides (LP3) were analyzed by size-exclusion chromatography combined with laser light scattering, viscometry, complex formation with Congo red, and atomic force microscopy. Their radii of gyration (<S2>z1/2) were 43.3, 62.6, 43.2 and 77.3 nm, exponents of <S2>z1/2 = k Mwv were 0.04, 0.50, 0.52 and 0.02, and intrinsic viscosities ([η]) were 9.945, 25.38, 308.2 and 452.1 mL/g, respectively. Moreover, the dependence of [η] on Mw was established to be [η] = 5.3 × 10−2Mw0.61 (mL/g). LPI had both a sphere-like conformation and a triple-helix structure, and LPII–IV existed as flexible chains. LP3, LPI, LPII and LPIII all exhibited direct inhibitory effects on A549, HeLa and HepG2 cells in a positive dose-dependent manner in the range of 50–400 µg/mL. The activities of LPIII, especially the inhibition of HepG2 cell proliferation, were stronger than those of others, which may be partly related to its flexible conformation. The present results support the cancer therapeutic potential of longan polysaccharides.
Share & Cite This Article
Yi, Y.; Huang, F.; Zhang, M.-W.; Zhang, R.-F.; Deng, Y.-Y.; Wei, Z.-C.; He, J.-R. Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp. Molecules 2013, 18, 11601-11613.View more citation formats
Yi Y, Huang F, Zhang M-W, Zhang R-F, Deng Y-Y, Wei Z-C, He J-R. Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp. Molecules. 2013; 18(9):11601-11613.Chicago/Turabian Style
Yi, Yang; Huang, Fei; Zhang, Ming-Wei; Zhang, Rui-Fen; Deng, Yuan-Yuan; Wei, Zhen-Cheng; He, Jing-Ren. 2013. "Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp." Molecules 18, no. 9: 11601-11613.
Notes: Multiple requests from the same IP address are counted as one view.